IMUNOLOGI KLINIK

(PCC-303)

Dosen pengampu:
Yudy Tjahjono, M.Sc. Biol. (STS)
Dr. Hendy Wijaya, M.Biomed (SAS(
 Sebelum UTS
Yudy Tjahjono, M.Sc
 Sebelum UTS
Yudy Tjahjono, M.Sc
 ATURAN PERKULIAHAN
STS
• DILARANG TITIP ABSEN
• QUIZ 30% DAN UTS 70%
• QUIZ 2x, akan diberitahukan kemudian
• KOMTING.

• SANKSI QUIZ: 10 ORANG TERJELEK

AKAN MENGHIBUR SELURUH KELAS
DENGAN LAGU DAN MUSIK.
 BAB 1

PENGANTAR

IMUNOLOGI
 Immune system

Innate Adaptive
(nonspecific) (specific)
1st line of defense 2nd line of defense

Cellular Humoral Cellular Humoral

components components components components
PROPERTY OF IMMUNE SYSTEM
 Development of the immune
system
Bone Marrow Thymus
Tissues
Stem cell
Granulocyte T cell

Myeloid Lymphoid
progenitor progenitor NK cell

Mast cell

B cell
Monocyte

2° Lymphoid
Macrophage
Dendritic cell Plasma Cell
 Cells of adaptive immune
system
• Lymphocytes
– B cells
• Plasma cells (Ab producing)
– T cells
• Cytotoxic (CTL)
• Helper (Th)
– Th1
– Th2
– Th17
– T-reg
 Cells of innate immune
system
• Phagocytes
– Monocytes/macrophag
es
– PMNs/neutrophils
• NK cells
• Basophils and mast
cells
• Eosinophils
• Platelets
Phagocytes – neutrophils
(PMNs)

• Characteristic
nucleus, cytoplasm
• Granules
• CD66 membrane
Neutrophil marker protein
• Chemotaxis

Geimsa stain
Source: www.dpd.cdc.gov
 Phagocytes – macrophages
• Characteristic nucleus
• lysosomes
Macrophage • CD14 membrane
marker protein

Source: Dr. Peter Darben, Queensland

University of Technology, used with permission
Natural killer (NK) cells
• Also known as large
granular lymphocytes
(LGL)
• Kill virus-infected or
transformed cells
• Identified by the
CD56+/CD16+/CD3-
• Activated by IL-2 and
IFN-γ to become LAK
cells
 Natural killer (NK) cells

NCR: Natural Cytotoxicity Receptor

Incorrect Pass: Incorrect MHC-I
Direct Kill
 Eosinophils
• Characteristic bi-
lobed nucleus
• Cytoplasmic
granules, stain with
acidic dyes (eosin)
– Major basic protein
(MBP)
– Potent toxin for
helminths
• Kill parasitic worms
Source: Bristol Biomedical Image Archive,
used with permission
 Mast cells
• Characteristic
cytoplasmic granules
• Responsible for burst
release of preformed
cytokines,
chemokines,
histamine
Source:
• Role in immunity
against parasites
Mast cells
 Dendritic cells
• Antigen presenting
cells
process antigen material
and present it on the
cell surface to the T
cells of the immune
system
Source: • present in those tissues
that are in contact with
the external
environment, such as
the skin and the inner
 Platelets / Thrombocytes
• stop bleeding by
clumping and clotting
blood vessel injuries
• Thrombus formation
• Platelet Adhesion

Source:
ANTIBODY
ANTIBODY

ANTIGEN

EPITOPE
 How antibodies binds
antigen?
ANTIGEN Recognition: SELF AND NONSELF

T-Cells B-Cells
 Antibody production:
Antibodies are made by B-Cells…….
but T-cells contain antibody-like
structure
Antigen
Binding Sites

T-Cells B-Cells

Cell Humoral
mediated
 Antibody production:
Antibodies are made by B-Cells.
Stem-Immature-Naive-Active-
Memory
T-Cells binds on APC

APC surface: Peptide Presenting

Molecule

MHC: Major histocompatibility

Complex
 Cell surface proteins which
essential for immune systems.
HLA: Human Leukocyte Antigen
 Cell mediated Immunity:
MHC proteins
“Self” Target
MHC-I MHC-II

Peptide from Peptide from

inside outside
Extracellular
intracelllular
 MHC-I
VIRUS ATTACK

VIRAL PROTEIN SYNTHESIS

Cytotoxin direct kills

Cell mediated immunities

T-KILLER
 MHC-II
Extracellular unknown particles: internalized in
Endosome/Lysosome of in B-Cells

Antibodies Production
Humoral immunities,
T-Helper
Adaptive response : Lymphocyte
T-Cells activated by APC
B-Cells
B-cells: Plasma and memory
Immune Response: Inflammation
Innate + Adaptive
 Next….
• Granulocyte Immunology…….