Rofp jLp pTkfbg

(Mushroom Spawn production)

 Rofp jLp pTkfbg
(Mushroom Spawn production)

 vfWffGg jfnL pTkfbg ug{sf] nflu jLpsf]

cfjZoQmf eP h:t} Rofp jLp pTkfbg ug{nfO{ klg
jLpsf] cfjZoQmf kb{5 . Rofpsf] :kf]/ g} o;sf] vf;
jLp xf] t/ o;jf6 jLp oTkfbg ug{ k|fljlws
b[li6sf]0fn] pko'Qm b]lv+b}g . :kf]/sf] ;f6f]
Rofpsf] /];fjf6 - Mycelium _ jLp ptkfbg ug{
;lhnf] x'g hfG5 . jLp pTkfbg ubf{ Mycelium
nfO{ ux'Fsf] jLp tyf cGo ljleGg dfWodx?df
a9fO{ jLpsf] ?kdf k|of]u ul/G5 . Rofpsf]
jLpnfO{ c+u|]hLdf Spawn elgG5 .
Rofpsf] jLp nfO{ ltg j6f steps df
pTkfbg ug]{ ul/G5
!=z'4 jLp -pure culture _ / o;sf] ;Djw{g
@=dfp jLp pTkfbg - Mother spawn _
#=Jofj;foLs jLp -5g]{ jLp_ pTkfbg -
planting spawn _
 !=z'4 jLp -pure culture _

 Rofpsf] z'4jLp cg';Gwfg s]Gb| tyf 7'nf Jofj;foLx?n] w]/}

nufgLdf pTkfbg ub{5g\ .
 ;fgf nufgLstf{nfO{ z'4 jLp pTkfbg ug{ jl9 vlr{nf] x'g]
ePsf]n] o:tf Jofj;foL x?n] 7'nf Jofj;foLx?jf6 jf ;+sng
s]Gb|jf6 jLp k|fKt ug'{kb{5 .
 ;+sng a]Gb|jf6 pknJw jLpnfO{ ljleGg sNr/ ldl8ofx?df
;Djw{g ug'{ kb{5 .
 Rofpsf] hft cg';f/ /];fnfO{ ;Djw{g u/L /fVgsf] nflu
lk=l8=P= -potato Dextrose Agar _ jf Pd=O=P= -Malt
Extact Agar _ jf l;=O=P= -Compost Extract agar _ sf]
k|of]u ug]{ ul/G5 .
@= Dffp jLp pTkfbg - Mother
Spawn production

 Dffp jLp pTkfbg ug{sf] nflu pure culture nfO{ klxnf

k]l6«Kn]6df x'sf{pg' kb{5 . o;sf] nflu k]l6«Kn]6nfO[{
!*) l8=;]=df @ #306f jf c6f]Sn]edf !@!l8=;]
tfqmddf @) ldg6 ;Dd lgd{lns/0f ug]{ .
 rlkª /8 tyf l:6n lg8n cflbnfO{ klg lgd{lns/0f ul/ ;f]sf]
;xfotfn] Rofpsf] /;fnfO{ sdfl6 lg8nsf] ;xfotfn]
cu/Kn]6df ;fl/G5 .
 Rofpsf] /];f ;fbf{ laminar flow sf] k|of]u ug'{ kb{5 .
qmdz
 Dffp jLp pTkfbg ug{sf] nflu pure culture nfO{ klxnf
k]l6«Kn]6df x'sf{pg' kb{5 . o;sf] nflu k]l6«Kn]6nfO[{
!*) l8=;]=df @–# 306f jf c6f]Sn]edf !@! l8=;]
tfqmddf @) ldg6 ;Dd lgd{lns/0f ug]{ .
 rlkª /8 tyf l:6n lg8n cflbnfO{ klg lgd{lns/0f ul/ ;f]sf]
;xfotfn] Rofpsf] /;fnfO{ sfl6 lg8nsf] ;xfotfn]
cu/Kn]6df ;fl/G5 .
 Rofpsf] /];f ;fbf{ laminar flow hood sf] k|of]u ug'{
kb{5 .
jLpsf] nflu pko'Qm dfWod
(Substrate)

 dfWod jgfpg rflxg] cfjZos ;fduLx?

M–
Rofpsf] jLp -Mother culture_ ,ux'Fsf] jLp ,
lk=l8=P=, k]6«LKn]6, skf;,ux'F ksfpg] efF8f],
bfljnf] ,PNo"ldlgod kmf]On, Sofnl;od
sfjf]{g]6, lhK;d, Knfl:6s y}nf, c6f]Sn]e, n]ldgf/
km|nf] x'8, rlkª /8 / l:6n lg9n cflb .
 dfWod (Substrate) sf] tof/L M–

 Rofpsf] /];f pTkfbg ug{sf] nflu ux'Fsf] bfgfnfO{

dfWod -substate _ sf] ?kdf k|of]u ul/G5 .
 ux'FnfO{ /fd|f]l;t s]nfO{,w'nf] ,d}nf,6's|f 6's|L tyf
sfl6Psf] bfgfnfO{ x6fpg] .
 ;kmf ux'nfO{ /ftel/ lr;f] kfgLdf lehfpg] / ef]nLkN6
!)–!% ldg6 ;Dd pl;Gg] . jLpnfO{ cfwf dfq kfSg] ul/
pl;Gg -ux'FnfO{ km'6g glbg] _ .
 cfwf kfs]sf] ux'nfO{ efF8fjf6 t'/Gt lemls lr;f]
kfgLn] /fd|f]l;t @–$ k6s kvfnL kfgL tsf{pg] / ;kmf
sk8fdf 5fofdf ;'sfpg] .
 ux'F rnfpgsf] nflu sf7sf] bfljnf]sf] k|of]u ug]{ .
qmdz
 jLpdf cln cln lr:ofg /xg] cj:yf x'Fb}df Sofnl;od sfjf]{g]6
– ^ u|f= / lhK;d – !@ u|f=k|lt lsnf] jLpsf] b/n] ld;fO[
/fVg] / ;j} bfgfdf kg]{ ul/ ldnfpb}
hfg] .
 ux'Fsf] bfgfsf] lk=Pr= &=) /fVg] .
 lgd{nLs/0f ug{sf] nflu ^–(OGrsf] Knfl:6ssf y}nfx?df k|lt
Knfl:6s @)) u|f=sf] b/n] ux'sf] jLp eg]{ / To;sf] d'vnfO{
kfgL g;f]:g] ul/ skf;n] jGb u/L PNo"ldlgod kmf]Pnn]
9fSg] .
 cj ux'F e/]sf] Knfl:6s y}nf]nfO{ c6f]Sn]edf !@! l8=;]=
tfks|ddf Ps 306f ;Dd jf c6f]Sn]edf @–$ l;l6 nfUg]
qmdz
 c6f]Sn]esf] k|];/ ;'Godf em/kl5 y}nfx?nfO{ jflx/ lgsfnL
lr;f] kfg]{ .
 jf]tn jf Knfl:6s y}nf lr;f] ePkl5 Laminar flow hood df /flv
Inoculation ul/G5 .
 /fd|f] Rofpsf] /];f ePsf] k]6LKn]6jf6 Inoculation ug{sf]
nflu #–$ j6f Rofpsf] /];fsf] 6'sf|x? chopping rod sf]
;xfotfn] sfl6 cu/ ;lxtsf] k]6«LKn]6df hLjf0f'/lxt jftfj/0fdf
;fl/G5 .
 k]6«LKn]6df ePsf] Rofpsf] /];fnfO{ y}nfdf ;f/]kl5
ux'F;+u ldnfpg #–$ k6s ;Dd xNnfpg' kb{5 .
qmdz
 /fd|f] Rofpsf] /];f ePsf] k]6LKn]6jf6 Inoculation ug{sf]
nflu #–$ j6f Rofpsf] /];fsf] 6'sf|x? chopping rod sf]
;xfotfn] sfl6 cu/ ;lxtsf] k]6«LKn]6df hLjf0f'/lxt jftfj/0fdf
;fl/G5 .
 k]6«LKn]6df ePsf] Rofpsf] /];fnfO{ y}nfdf ;f/]kl5
ux'F;+u ldnfpg #–$ k6s ;Dd xNnfpg' kb{5 .
#= Jofj;foLs jLp -5g]{_ pTkfbg
(Planting Spawn)M–

 Jofj;foLs jLpsf] nflu klg ux'Fsf] dfWod - substrate _

jgfpg] tl/sf – dfp jLpdf h:t} ul/G5 .
 ux'Fsf] dfWod jgfO{ ;s]kl5 inoculation ug{ k]6«LKn]6sf]
/];fo'Qm cu/sf] dfp jLpsf] k\of]u ul/G5 .
 Pp6f Knfl:6s y}nf jLpnfO{ inoculation ug{ @–# rDrf dfp
jLpsf] cfjZoQmf kb{5
 Inoculation ubf{ chopping rod , ;'O/f] / rDdr hlxn] klg
lgd{lns/0f u/]sf] x'g k5{ / dfp jLpsf] y}nf / 5g]{ jLpsf]
y}nfnfO{ d'v hf]8]/ lj:tf/} dfp ljhgsf] y}nfjf6 @–# rDdr
/];f ;lxtsf] ux'F ;fg' k5{ .
qmdz
 jLp ;fg]{ sfo{ Laminar flow hood df hLjf0f'/lxt jftfj/0fdf
ug'{ kb{5
 To;kl5 y}nfx?nfO{ skf;sf] ljsf]{ nufO{ PNo"ldlgod
kmf]Onn] ljsf]{ 9fsL /j/n] jfFWg' kb{5 / incubation df
/fVg' kb{5 .
 jLpnfO{ incubation df /fVbf Knfl:6s y}nfdf jLpsf] hft ,
cf]yf/f] u/]sf] ldlt n]v]/ /fVg' kb{5 .
 Ps kf]sf dfp jLpn] @)–@% kf]sf tof/ ug{ ;lsG5 .
 o;/L jLp cf]yf/f] /fv]sf] @–# xKtf leqdf Rofpsf] /];f ;j}
ux'Fdf km}lng hfG5 / Rofp ljs|L ug{ of]Uo x'G5 .
 jLpsf] e08f/0f M–

 Rofp jLpnfO{ tof/ ePkl5 t'/Gt} k|of]u ug{

pko'Qm x'G5 .
 o;nfO{ e08f/0f ug{ @–$ l8=;]=tfks|d ;j}
eGbf pQd dflgG5 / o;nfO{ dlxgf lbg;Dd
e08f/0f ug{ ;lsG5 .
 jLp KoflsË tyf 9'jfgL M–

 Rofp jLpnfO{ lbgdf eGbf /ftdf 9'jfgL

ug{ pko'Qm x'G5 .jLp KoflsË ug{ k"j{
skf;sf] ljsf]{ l7s 7fs 5 5}g r]s ug'{ kb{5 /
jLpnfO{ sf6'{gdf KoflsË
u/]/ dfq 9'jfgL ug' kb{5 .
s]lx kmf]6f]x?
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