NMR Services

NMR spectroscopy is a key analytical technique for

structure elucidation of a wide range of materials
from small molecules to compounds. The technique
provides detailed molecular information that allows
researchers have in-depth understanding of
composition, chemical structure, morphology, and
dynamics. NMR is particularly useful in analysis of
pharmaceuticals, screening weak-binding
compounds and developing into drug-like inhibitors
for drug discovery.
Supported by our NMR platform, Creative Biostructure
offers high quality customized services ranging from
production of labeled-proteins to acquisition and analysis
of high-field NMR data for researchers in science and
pharmaceutical industry.
NMR workflow
 NMR services capabilities

 Compound structure determination

 Chemical composition analysis
 Molecular characterization
 Reaction kinetics examination
 Quality control
 Dynamics, disorder analysis
 Isotope-labeled protein production
 Quantitative analysis
 Drug screening and design
 NMR mapping for living cells
 Mapping structure of biological drugs by NMR
 In vivo magnetic resonance imaging
 NMR-based pharmacometabonomics
 Stable isotope labeling for nucleic acids
 Combining NMR and mass spectrometry for metabolomics
 Combining X-ray crystallography and solution NMR data
 NMR analysis for biopharmaceutical development
Why Creative Biostructure?

State-of-the-art instruments
Senior scientists with extensive exerience
Rapid turnaround time
 Sample preparation guideline
Labeling with isotopes: Protein NMR spectroscopy requires
isotope enrichment, such as 15N, 13C, 31P, 19F in common.
Samples with other isotope are also acceptable.
 Size: The molecular weight of biomacromolecule of a NMR
sample is typically less than 50 KDa for high-resolution
structure elucidation. Larger proteins or complexes can be
studied to identify ligand/drug-binding site or to characterize
the physical state.
 Amount: For solution NMR, a sample typically contains 1-10
mg/ml or 0.3 mM purified protein in a suitable buffer; for solid-
state NMR, we require larger amount of samples, please talk to
our specialist for more information.
 Stability: For most in-depth studies by NMR, the protein
needs to be stable for several days at room temperature for
data acquisition.
 Thank you !
Excerpt from:
https://www.creative-
biostructure.com/nmr-services_28.htm