Membrane proteins

Cell membrane functions as a boarder of cell by

separating it from the outside environment, and the
membrane proteins are the gateways to the cell.
Nutrients, ions, waste products, even DNA and
proteins are transported into and out of cells by
membrane proteins. Membrane proteins involve in
many cellular functions including: signaling
transduction, cell adhesion, cell motility,
transportation, and cell communication. Given their
important roles in cell functions, membrane proteins
are often considered to be perfect drugs targets.
In fact, about two-thirds of approved drugs target
membrane proteins, nevertheless, these account for only a
very small part of the potential targets. Therefore,
membrane protein is still a main focus for new drug
discovery.
Challenges for membrane production and characterization

nvestigating membrane protein structure and function can

provide valuable information for drug characterization and
optimization, however, such proteins are inherently difficult to
purify and characterize. Even when expressed at high levels,
the purification can still be challenging. Generally, cellular
expression of membrane protein can lead to aggregation and
malfunction due to the hydrophobic properties of the
transmembrane domain. Detergents are used to stabilize the
membrane proteins during purification. But these detergents
bring other issues. Detergents with stabilizing power may
impair protein crystallization, and they may also contribute to
poor sensitivity in NMR and X-ray crystallography studies.
Strategies for membrane protein production

Creative Biostructure membrane protein platform has

developed several efficient membrane protein
expression systems and protein purification
approaches to handle the dilemmas talked above.
These expression systems and purification approaches
are listed below:
Membrane protein expression systems:

Cell based membrane protein expression

Cell free membrane protein expression
(CFPS)
Protein detergent-free expression
Animal free recombinant protein
expression
Plasma membrane protein isolation
Protein Purification
Chromatography
TFF/Diafiltration
Native protein purification

Following optimal expression, purification and

crystallization of your protein of interest, Creative
Biostructure guarantees precise determination of the
native target structures with medium or high
resolution. Based on thorough structural analysis, we
further offer antibody development, drug discovery
and vaccine formulation developments.
Customized membrane protein services
As a leader in membrane production, Creative
Biostructure provides membrane protein services
from gene to structure with an emphasis on protein
purification, crystallization, structure determination
and analysis. Services include, but not limited to:
 Protein domain prediction
 Plasmid design and construction
 Protein expression
 Protein purification
 Protein process development and manufacturing
 Thank you !
Excerpt from:
https://www.creative-biostructure.com/custom-
membrane-protein-services_25.htm