Virus-like

particle service
The formation of virus-like particles (VLPs) is a result of self-
assembly of one or several types of viral structural proteins, such as
Envelope or Capsid. The component proteins can originate from
different viruses or be recombinantly expressed. Various systems
have been used in the successful construction of VLPs from different
sources including bacterial, yeast, insect cells, plant and mammalian
hosts. VLPs are non-infectious because they contain no viral genetic
material. VLPs include a number of biological objects, such as
uncharacterized structures with viral morphology, empty structures
of viral origin, self-assembled gene products, etc. VLPs also provide
a highly efficient vaccine platform and a wide variety of VLPs have
entered pre-clinical and clinical development.
Lipoparticles are specialized VLPs that incorporate high
concentrations of target membrane proteins in their native
conformation. A lipoparticle usually consists of a retroviral
core protein (Gag) and a conformational-complex membrane
protein, co-expressed, assembled and released from host
cells. Gag core proteins self-assemble at the plasma
membrane, where they bud off and capture target membrane
proteins. Since the membrane proteins within lipoparticles
are derived directly from the cell surface without mechanical
disruption or detergents, the native structure and orientation
of the membrane proteins are retained.
Schematic diagram of the lipoparticle
Lipoparticles have many advantages in isolating membrane
proteins: firstly, they are homogeneous and physically well
defined, capable of maintaining the native structures of target
membrane proteins in high densities; secondly, they can be
used to detect membrane protein interactions and therefore
have wide applications in antibody screening, phage and
yeast display, immunogens production and ligand binding
assays.

Creative Biostructure uses MemPro™ virus-like particles (VLPs)

to efficiently isolate membrane proteins in the form of
lipoparticles.
Ordering Process
 Thank you !
Excerpt from:
https://www.creative-biostructure.com/vlp-construction-40.htm