Data Processing

Creative Biostructure now can provide a collection of tools and

analysis package for biomolecular structure determination,
refinement and analysis from crystallographic, EM or NMR data. In
structural biology field, in order to reveal a dedicated and reliable
structure of biomolecule, research data is acquired, interpreted,
published, reused, and sometimes eventually discarded. Creative
Biostructure helps the development of appropriate infrastructural
services, ones which make it easier and time-saving for researchers
to collect, read and process the data and obtain the expected and
target structure results.
Project Objectives
With this deliverable, the project has reached or the deliverable has contributed
to the following objectives, including but not limited to:
Crystallographic data processing and structure determination

The physical infrastructure for X-ray crystallography structural biology includes

synchrotrons, which are affordable by a nation. There are 47 in the world
(lightsources.org). Each synchrotron provides a number of beamlines for
experiments, usually including several optimized for macromolecular X-ray
crystallography, often some for other structural biology techniques including
SAXS (Small-Angle X-Ray Scattering) and CD (Circular Dichroism), and usually
some beamlines for material sciences and other non-biological applications.
Creative Biostructure has access to the most modern and popularly used
synchrotron X-ray resources, such as ALS, SSRL, CAMD, Photon Factory,
SSRF, etc.
EM data processing and interpretation

Improvements in microscopes, direct electron detectors,

and processing software have led to a rapid increase in the
number of high resolution cryoEM structures – the
“resolution revolution”. This has led in turn to significant
investments in electron microscopes around the world.
Structural biologists in Creative Biostructure are working on
harder targets: fewer single proteins, larger
macromolecular assemblies, more membrane proteins.
shows the increasing proportion of PDB entries belonging to these more difficult categories.
NMR data processing and interpretation
Creative Biostructure has access to the Structural Biology NMR
Resource to obtain high-resolution solution NMR spectrometers ranging
from 300 MHz to 900 MHz in field strength. These are used for small
molecule analysis and for studies of macro-molecular structure,
dynamics, and function. Our highly trained associates assist in the
collection and interpretation of routine data, for training in instrument
usage, for performing data processing and interpretation and to partner
for more extended collaborative studies of protein or nucleic acid
structure and dynamics.

1485
Thank you !
Excerpt from:
https://www.creative-biostructure.com/Data-
Processing-and-Interpretation-584.htm