Documente Academic
Documente Profesional
Documente Cultură
From Fermentation
to Inclusion Bodies
Group I
Raymond Christopher Setiawan, Hong-Yuan Chen,
Shu-Han Wang, Anding Hsia, Bing-Jun Hsu
OUTLINE
2
Some advices we’ve got
Week 1 2 3
∗ ′
𝑘𝑡𝑟𝑎𝑛𝑠𝑓𝑒𝑟 𝐶𝐷𝑂 − 𝐶𝐷𝑂
𝑋𝑂2 =
𝑞𝑂2
Agitator speed and impeller design
∗ ′
𝑘𝑡𝑟𝑎𝑛𝑠𝑓𝑒𝑟 𝐶𝐷𝑂 − 𝐶𝐷𝑂
𝑋𝑂2 =
𝑞𝑂2
Volumetric flow rate (for 30 m3 batch) :
𝑚3 1992𝑚3 𝑂𝑥𝑦𝑔𝑒𝑛 𝑠 1
30 3600
𝑏𝑎𝑡𝑐ℎ 𝑚3 𝑏𝑟𝑜𝑡ℎ − ℎ ℎ 0.21𝑚3 𝑂𝑥𝑦𝑔𝑒𝑛
𝑚3 𝑎𝑖𝑟
3
= 79.04 𝑚 𝑎𝑖𝑟/𝑠
High cell density media for Escherichia coli are generally designed
for aerobic cultivations – consequences for large-scale
bioprocesses and shake flask cultures. Soini et.al. 2008. 6
Microbial Cell Factories. Vol 7 p 26.
Stainless Steel selection
8
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