Progress Report

From Fermentation
to Inclusion Bodies
Group I
Raymond Christopher Setiawan, Hong-Yuan Chen,
Shu-Han Wang, Anding Hsia, Bing-Jun Hsu
OUTLINE

What we have What we had Next Project

got from the done these
factory visit two weeks

2
Some advices we’ve got

1. The strand need to be tested in small laboratory for effectiveness

Response: Due to the nature of our design, we will first assume that
our strain has the highest possible yield.

2. One batch usually has to be carried for 5 to 7 days.

Response: If so, we will set our number of batches as 50 per year. One
batches will produce approximately 2 kg or insulin.

Batch 3 Batch 2 Batch 1 finished

Week 1 2 3

3. E. coli need to be constantly be aerated with oxygen and be

monitored closely for its cell density
Response: We had managed to calculate the theoretical oxygen needs
for E. coli, we will show them in the final paper. 3
 C. Chen Ching et.al. (1983), Unit Operation Laboratory
Student Guides, National Taiwan University, New Wun
Bioreactor design Ching Developmental Pub. Co., Ltd.

Aeration is needed to support aerobic fermentation

∗ ′
𝑘𝑡𝑟𝑎𝑛𝑠𝑓𝑒𝑟 𝐶𝐷𝑂 − 𝐶𝐷𝑂
𝑋𝑂2 =
𝑞𝑂2
Agitator speed and impeller design

We will use COMSOL to determine the model

𝑃02 𝑁𝐷𝑎3 0.45

𝑃𝑜 = 0.7𝑁 3 𝐷𝑎2 𝑉 𝑃𝑔 = 𝑘( 0.56 )
𝑄 4
 The progress of our project

Oxygen Consumption Rates of Bacteria under Nutrient-Limited

Conditions. Riedel et.al. 2013. J. Applied and Env. Microbiology.
5
79(16): 4921–4931
 https://chemistry.stackexchange.
Optimal DO for fermentation is above 30%. com/questions/66727/solubility-
of-oxygen-in-water-with-
temperature-why-does-the-
change-in-solubilit

∗ ′
𝑘𝑡𝑟𝑎𝑛𝑠𝑓𝑒𝑟 𝐶𝐷𝑂 − 𝐶𝐷𝑂
𝑋𝑂2 =
𝑞𝑂2
Volumetric flow rate (for 30 m3 batch) :

𝑚3 1992𝑚3 𝑂𝑥𝑦𝑔𝑒𝑛 𝑠 1
30 3600
𝑏𝑎𝑡𝑐ℎ 𝑚3 𝑏𝑟𝑜𝑡ℎ − ℎ ℎ 0.21𝑚3 𝑂𝑥𝑦𝑔𝑒𝑛
𝑚3 𝑎𝑖𝑟
3
= 79.04 𝑚 𝑎𝑖𝑟/𝑠

High cell density media for Escherichia coli are generally designed
for aerobic cultivations – consequences for large-scale
bioprocesses and shake flask cultures. Soini et.al. 2008. 6
Microbial Cell Factories. Vol 7 p 26.
 Stainless Steel selection

Type Difference Corrosion Note

resistance
304 No Worse 304 can withstand corrosion from most oxidizing acids.
Molybdenum it is susceptible to corrosion from chloride solutions
316 With Better Resistant to acid (chloride), and other industrial
Molybdenum solvents
https://www.reliance-foundry.com/blog/304-vs-316-stainless-
steel#gref

Tank Turnover is a measure of how

effective a mixer’s pumping rate
is relative to turning a batch over
a particular number of times a
minute

i.e. Vol of one tank: 30 m3

TOR: 5
Mixer pumping rate: 600 m3/min 7
 What we will do this week

1. We will try to try designing our Process in ASPEN this

Thursday.

2. We will give our data to be given to Group III for

economic Analyses.

8
THANK YOU