Producción de insulina

a partir de
Escherichia Coli
Ingeniería
genética
2
3
Escherichia Coli
4
¿Porqué E. Coli?
1. Su genoma es conocido desde hace varios años, lo que amplía considerablemente
las posibilidades de su manipulación genética
2. Existe una gran cantidad de conocimiento acumulado sobre su fisiología y
metabolismo
3. Se tienen varios vectores bien establecidos para la producción de PR
4. Puede crecer rápido en medios muy simples, con altos niveles de producción de
PR.
ESTRUCTURA
DEL MO
CARACTERISTICAS

1. Bacilo Gram negativo.

2. Móviles (flagelos perítricos).
3. Miden 0.5 µ de ancho por 3 µ de largo.
4. Catalasa positivos.
5. Respiración anaerobia: Oxidasa negativos, reducen nitratos a nitritos.
6. Producen vitamina B y K.

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1. Bacilo Gram negativo

9
1. Bacilo Gram negativo

10
2. Móviles (flagelos perítricos).

11
2. Móviles (flagelos perítricos).

12
2. Móviles (flagelos perítricos).

13
CONDICIONES DE
CRECIMIENTO
14
CONDICIONES DE CRECIMIENTO
1. Temperatura del entorno: 35ºC – 43º C
2. Tiempo de reproducción: 20 minutos
3. Temperatura limite de crecimiento: 7ºC
4. Temperatura de eliminación: 70ºC
5. pH: 7,2
6. pH para detener el desarrollo: inferiores a 3,8 superiores a 9,5
7. Actividad de agua: 0,99
8. Actividad de agua para detener el desarrollo: inferiores a 0,94

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METABOLISMO
16
METABOLISMO

1. Ruta metabólica de respiración anaerobia (desnitrificación)

2. Ruta metabólica de respiración aerobia
3. Metabolismo del acetato.
4. Metabolismo de maltosa.
5. Crecimiento in vitro con diferentes tipos de azucares.

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1. Ruta metabólica de respiración anaerobia (desnitrificación)

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1. Ruta metabólica de respiración anaerobia (desnitrificación)

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2. Ruta metabólica de respiración aerobia

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3. Metabolismo del acetato.

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4. Metabolismo de la maltosa.

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5. Crecimiento in vitro con diferentes tipos de azucares.

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METABOLISMO
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In two or three columns
Yellow Blue Red
Is the color of gold, Is the colour of the Is the color of
butter and ripe clear sky and the blood, and because
lemons. In the deep sea. It is of this it has
spectrum of visible located between historically been
light, yellow is violet and green on associated with
found between the optical sacrifice, danger
green and orange. spectrum. and courage.

25
A picture is worth a
thousand words
A complex idea can be
conveyed with just a single
still image, namely making
it possible to absorb large
amounts of data quickly.

26
Want big impact?
Use big image.

27
Use diagrams to explain your ideas

Lorem ipsum
tempus

Lorem ipsum congue

tempus

Lorem ipsum
congue
tempus

28
And tables to compare data

A B C

Yellow 10 20 7

Blue 30 15 10

Orange 5 24 16

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Maps

our office

30
89,526,1
24
Whoa! That’s a big number, aren’t you
proud?

31
 89,526,124$
That’s a lot of money

185,244 users
And a lot of users

100%
Total success!

32
 Our process is easy

1 2 3

33
 Let’s review some concepts
Yellow
Is the color of gold, butter
and ripe lemons. In the
spectrum of visible light,
yellow is found between
green and orange.
Blue
Is the colour of the clear sky
and the deep sea. It is
located between violet and
green on the optical
spectrum.
Red
Is the color of blood, and
because of this it has
historically been associated
with sacrifice, danger and
courage.

BlueYellow Red
Is theof
Is the colour color
the of gold,
clear skybutter Is the color of blood, and
and theand
deepripe
sea.lemons.
It is In the because of this it has
spectrum
located between violetof visible
andlight, historically been associated
yellow is
green on the optical found between with sacrifice, danger and
green and orange.
spectrum. courage.

34
You can insert graphs from Google Sheets

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Mobile project
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Tablet project
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Desktop project
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38
Thanks!
Any questions?

You can find me at:

@username
user@mail.me

39
Credits
Special thanks to all the people who made and
released these awesome resources for free:
༝ Presentation template by SlidesCarnival
༝ Photographs by Unsplash

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 Presentation design
This presentation uses the following typographies:
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