THE EFFECTIVENESS OF COLEUS (Plectranthus

seutellarioides) EXTRACT AS AN ALTERNATIVE pH

INDICATOR TEST STRIP IN THE URINE SPECIMEN
 THE RESEARCHERS:

Chelsea Ann Alayon Shieliza Alonsabe

Paula Rizz Baterna Fedser Reuel Sandico Glenneth Mae Villaflor

 INTRODUCTION
Coleus plant (Plectranthus seutellarioides)
also known as mayana or lampunaya plant
in the Philippines.

It comes in variety of colors like yellow, red,

pink, green and purple.

Coleus plant is rich in anthocyanin because

of its color.
 INTRODUCTION
We are going to observe the
effectiveness of coleus plant extract
as a pH indicator strip in urine
specimens.

 Coleus plant extract will be use in

making pH indicator solution.
 OBJECTIVES
General objectives:
This study will tend to determine the
effectiveness of coleus (Plectranthus
seutellarioides) extract as a pH indicator
test strip in various urine specimens.
 OBJECTIVES
SPECIFIC OBJECTIVES:
Determine the acidity or alkalinity of
various urine specimens and its
corresponding condition, using coleus
(Plectranthus seutellarioides) extract as pH
indicator.
Determine the effectiveness of anthocyanin
as an alternative for acid-base testing.
 CONCEPTUAL FRAMEWORK

• Concentration of pH
COLEUS (Plectranthus in urine specimen
seutellarioides) extract • Color of pH indicator
test strip

INDEPENDENT VARIABLE DEPENDENT VARIABLE

 HYPOTHESIS OF THE STUDY

There is no significant effect on the coleus

(Plectranthus seutellarioides) extract as a
pH indicator test strip in various urine
specimens.
 SIGNIFICANCE OF THE STUDY
This study will be deemed important to
the following people, group &
organization:

Pharmaceutical companies - This

may give insightsand potential
pharmaceutical ingredients that give
various beneficial health effects.
 SIGNIFICANCE OF THE STUDY

 Patients - this will serve as basis

for prompt and accurate
medications for conditions
concerning homeostatic regulation
of the pH in the body’s
extracellular fluid.
 SIGNIFICANCE OF THE STUDY

Community - this study will benefit the

populace in terms of imparting knowledge for
home remedies and providing awareness of
acid-base homeostasis by testing urine sample.
 SIGNIFICANCE OF THE STUDY

Health personnel/ health sectors

- the outcome of the study will be
helpful to update the profile of the
coleus species as a pH indicator.
 SIGNIFICANCE OF THE STUDY

Future researchers -this may give assistance

of support as to hint to procedures and basis for
the further studies in the near future and in
providing additional information as an alternative
pH indicator test.
 SCOPE AND LIMITATION
This study will focus on the effectiveness of coleus
(Plectranthus seutellarioides) extract as a pH indicator test
strip in the urine specimen. The researcher will observe if
coleus (Plectranthus seutellarioides) can use as a pH
indicator strip after doing some experiments and test. This
result will help the researchers determine if coleus
(Plectranthus seutellarioides) would make a successful pH
indicator.
 SCOPE AND LIMITATION
All investigatory activities and
laboratory works will be conducted at
science laboratory at Riverside College
Inc., On February 2019.
 RESEARCH METHODOLOGY
RESEARCH DESIGN
This study is an experimental research
emphasizing on the effectiveness of coleus’
anthocyanin property on indicating urine’s
ph.
 RESEARCH METHODOLOGY
Collection and preparation of the extract and
solution
The researchers will be collecting coleus
leaf.
In the preparation of coleus leaf indicator
solution, 1kg of coleus leaf, put it in a
blender and add 500ml of boiling water.
Blend the water and coleus leaves.
 RESEARCH METHODOLOGY
Collection and Preparation of the extract and
solution
 Keep the mixture blending until the water is
purple.
Transfer the mixture into a pan (choose a
container that is stain resistant) and pour the
mixture through a strainer to remove any pieces
of leaf from the indicator solution.
 RESEARCH METHODOLOGY

Collection and Preparation of the extract and

solution
Add 50mL of isopropyl alcohol to the solution to
protect from bacterial growth.
 The alcohol may alter the solution, if this
happens, add vinegar until the solution goes
back to red purple.
 RESEARCH METHODOLOGY
Collection and Preparation of the extract and
solution
Prepare an acid free paper and then soak it into
the solution.
 Allow paper to air dry on a towel leave it
overnight. Cut the paper into strips.
Put strips inside a dry airtight container and
place it in a cool area.
 RESEARCH METHODOLOGY
PROCEDURE
Put strips inside a dry airtight container and
place it in a cool area
The pH indicator strip will be used to
indicate the urine’s ph.
Collect the specimen (urine) needed in a
test tube up to its neckline.
 RESEARCH METHODOLOGY

Procedure:
Test strip will be soaked for 1 second in
the urine specimen.
For a few seconds observe for the
change color in the strip. Each color has
a corresponding pH result.
SCHEMATIC DIAGRAM OF METHODOLOGY

Extraction by Preperation of Indicator

Coleus leaf
Concortion solution and Acid free
paper

Observation of pH Testing urine’s pH using

corresponding the alternative pH
Color change indicator strip
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THANK YOU!!!