Biosynthesis of Silver Nanoparticles

remediated by Sansevieria zeylanica

Willd. and Comparative Evaluation of its
Toxicity and Antimicrobial Activities to
its Leaf Extract
Shahri Bin Rendon
Chemistry 198 – Methods of Chemistry
Research (Proposal defense)
Mindanao State University
Marawi City 9700 Phillipines
 THIS STUDY

Diseases
Nanoparticles

Plants
Statement of the problem:
The following questions must be answered:

 Can remediation of the plant leaf of Sansevieria

zeylanica Willd. biosynthesize silver nanoparticles using
its extract?

 Does synthesized silver nanoparticles of Sansevieria

zeylanica Willd. exhibits cytotoxic properties in
comparison to its extract alone?

 Does growth of the tested bacteria and fungi be

inhibited by the biosynthesized silver
nanoparticles in comparison to the plant extract alone?

 What is the morphology and size of the biosynthesized

silver nanoparticles?
Objectives of this study:
Specifically this study aims to:

 Biosynthesize silver nanoparticles remediated by Sansevieria

zeylanica Willd.

 Observe the color change and the production of silver

nanoparticles being for maximum of 30 min under UV-Vis
Spectrometry.

 Monitor and evaluate the toxicity activity exhibited by silver

nanoparticles in four different concentrations (25%,
50%, 75% and 100% (v/v)) In comparison to its extract alone
for 0, 24, 48 and 72 h.
Continued….
 Evaluate the antimicrobial activity of the biosynthesized silver
nanoparticles against Gram (-) negative bacteria (E. coli), Gram
(+) bacteria (S. aureus) and fungi (C. Albicans, and A. niger).

 Monitor and evaluate the progress of production silver

nanoparticles after 0, 24, and 48 hours of reaction using UV-Vis
spectrophotometry.

 Based on the changes of their characteristic IR peaks using FTIR

spectroscopic method, we are to know the possible functional
groups responsible in the reduction of silver cations to neutral
silver atom.

 Determine the morphology and shape of the silver-

nanoparticles produced using Scanning Electron Microscopic-
Energy Dispersive X-Ray (SEM-EDS).
Significance of the study:
• Green, eco- and environmental friendly
new route determination for the
synthesis of silver nanoparticles

• Nanomedicine inovation

• Improvement of nanoscale technologies

Scope and Limitation:
 Green Chemistry principles

 Nanobiotechnology

 Toxicity exhibition using seed germination toxicity test

 Antimicrobial Activity determination using paper disk

diffusion method

 Characterization under UV-Vis Spectrophotometry,

Scanning Electron Microscopy (SEM) and Fourier
Transform Infrared (FTIR) Spectroscopy
 Related
Literature
Nanoparticles
Khushdil Khan*, Tarun Gupta, Bhawna Dangi,
Nitin Jain and Gaurav Sharma
Department of Biotechnology
Mohanlal Sukhadia University, Udaipur
(Raj.),INDIA

“GREEN SYNTHESIS OF SILVER

NANOPARTICLES AND THEIR
ANTIMICROBIAL ACTIVITY: A
REVIEW”
 have one dimension that measures 100 nanometers
or less

 have a greater surface area per weight than larger

particles which causes them to be more reactive to
some other molecules

 Color – Nanoparticles of yellow gold and gray silicon

are red in color

 Silver nanoparticles melt at much lower temperatures

(~115 °C for 2.5 nm size) than the silver slabs (1064
°C)

 since the particles are smaller, they absorb greater

amount of solar radiation
 Green Synthesis of Silver Nanoparticles
Mechanical or ball milling
Chemical etching Toxic
Thermal/ laser ablation
Sputtering

Synthesis of Chemical/Electrochemical precipitation

nanoparticles Vapour deposition
Atomic/molecular condensation
Sol-gel process
Spray pyrolysis
Laser pyrolysis
Aerosol pyrolysis

Green synthesis
Figure 3.Different Approach of synthesis of -Bacteria Non
nanoparticles modified from “A review on plants -Plant toxic
extracts mediated synthesis of silver -fungus
nanoparticles for antimicrobial application: A
green Expertise by S. Ahmed et. al., 2016
Antimicrobial Properties of Silver
Nanoparticles
S. Rajeshkumar*, L.V. Bharath

“Mechanism of plant-mediated
synthesis of silver nanoparticles e A
review on biomolecules involved,
characterization and antibacterial
activity”
 The antibacterial activity of AgNPs can be
characterised in two types: the inhibitory
action and biocidal action.

 The AgNPs antibacterial activity strongly

depends upon the various factors including
pH, temperature, type of bacteria, AgNO3
concentration.

 Mahendra has been reported that the AgNPs

preferably attack the bacterial respiratory
chain, cell division eventually leading to cell
death
 Description
70 Species
Properties

Sansevieria zeylanica Willd.

Uses
Methodology
1. Sample Collection
 Rinsed thoroughly with tap water or faucet
water followed with distilled water

 Air dry

 Finely incised

 Decocted for 20 min

 Filter with Whatman No. 1 thrice

 Refrigerate under 4℃ for further experiments

 2. Plant Extract

Plant Extract

Toxicity Test Addition of silver nitrate

solution (AgNO3)

Silver nanoparticles
Antimicrobial Assay (AgNPs)
 3. Synthesized Silver Nanoparticles

Synthesized silver Antimicrobial

Toxicity test nanoparticles Assay

Fourier Transform
UV-Vis Characterization Infrared
Spectroscopy
Spectroscopy

Scanning Electron
Microscope
 Seed Germination Toxicity Test

Plant Extract Silver Nanoparticles

25% 50% 75% 100%

• 50 seeds will
be put

• Kept in the
dark for
germination
 Paper disk diffusion test

Plant Extract Silver Nanoparticles

Microbes
used Gram (+) Gram (-) Fungal Fungal
Legend:

Plant Extract

Silver nanoparticles

Double Distilled
water
Gram (+) Gram (-) Fungal Fungal

Incubated
Legend:

Plant Extract

Silver nanoparticles

Double Distilled
water

Zone of inhibition
Data Evaluation
• Seed Germination Toxicity Test
• The final germination percentage was
calculated based on total number of
germinated seeds at the end of
experiment. The measurements were
carried out according to International
Rules for Seed Testing. Germination
indices were calculated using the following
equations.
• Germination percentage (GP %) = (Gf/n) × 100
where, Gf is the total number of germinated seeds at
the end of experiment and n is the total number of
seed used in the test.

• Mean Germination Time (MGT) = Ʃ NiDi/n

where, Ni is number of germinated seeds till ith day
and Di is number of days from start of experiment till
ith counting and n is total germinated seeds.

• Germination rate (GR) = Σ Ni/ΣTiNi

where, Ni is the number of newly germinated seeds
at the time of Ti.
= (a/1)+(b-a/2)+(c-b/3)+.....+(n-n-1/N)
Statistical analysis

• Means and standard deviations were

derived from measurements on three
replicates for each treatment and the
related controls. The data obtained from
the various treatments were statistically
analysed using the t-test at a significance
level of 0.5.
References
• Chung et al. Plant-Mediated Synthesis of Silver Nanoparticles: Their Characteristic
Properties and Therapeutic Applications Nanoscale Research Letters (2016) 11:40

• Banerjee et al. Leaf extract mediated green synthesis of silver nanoparticles from widely
available Indian plants: synthesis, characterization, antimicrobial property and toxicity
analysis Bioresources and Bioprocessing 2014, 1:3

• P. Heera and S. Shanmugam (2015). Nanoparticle Characterization and Application: An

Overview. Int.J.Curr.Microbiol.App.Sci (2015) 4(8): 379-386

• S. Ahmed et. al., (2016) A review on plants extract mediated synthesis of silver
nanoparticles for antimicrobial applications: A green expertise. Journal of Advanced
Research (2016) 7, 17–28

• V. Mali et. al., (2018). Green Synthesis of Silver Nanoparticles from Plants. Proceeding of
International conference on Advances in Materials Science ISBN 978-93-5254-490-5347

• VJ Mohanraj and Y Chen (2006). Nanoparticles – A Review., Tropical Journal of

Pharmaceutical Research, June 2006; 5 (1): 561-573

• Zainab M. Almutairi et. al., (2015). Effect of silver nanoparticles on seed germination of
crop plants. JOURNAL OF ADVANCES IN AGRICULTURE vol .4, no.1
 END OF
PRESENTATION
Sansevieria ballyi Dwarf
Sansevieria Cleopatra Snake Plant
Sansevieria ehrenbergii Blue
Sansevieria trifasciata Golden Hahnii
Golden Birds Nest
Sansevieria trifasciata Twisted Sister Twisted
Sister Snake Plant
Sansevieria trifasciata Hahnii Birds Nest
Sansevieria
 Sansevieria zeylanica Willd
• Is a herbaceous,
succulent,
perennial plant,
growing to a height
of 90 cm. Leaves
from a basal
rosette, are flat,
thick, leathery,
sword-shaped
variegated with
greyish white
transverse
markings.
• Flowers are whitish
green up to 5 cm
long
Properties
Detoxifying
Heat-clearing
Insecticidal
Analgesic
Antipyretic
Air-purifying properties
Anti fungal properties
Uses
• Folkloric
– No recorded folkloric use in the Philippines.
– In China, Decoction used for detoxification as
anti inflammatory and treatment for sores
and snakebites.
– Also use for boils, cough, bronchitis,
traumatic injuries
– In Sri Lanka, rhizomes used for coughs and
colds, leaves used for snakebites
• Medicinal Use
– Dried rhizomes and roots provide material
for antiseptic ointment
– The roots are used for purgative, tonic,
expectorant and anti fever remedies
– Used traditionally in the treatment of septic
wounds
• Other uses
– Leaves used for making mats, sails, cordage,
coarse cloth and the production of paper
pulp Slide 14