“Preparation and biocompatible evaluation of

sugarcane bagasse cellulose nanofiber incorporated

starch based bioedible film’’

Medini Mohan Chetia

Department of Physics
Under the supervision of Dr. Kshirod K. Dash.
(Department of Food Enginering and Technology)
Tezpur University

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 Sugarcane Bagasse

Extraction of

Juice

Harvested sugarcane Sugarcane Bagasse

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• Sugarcane Bagasse (SCB) is the end product left after the juice
has been extracted from sugarcane.

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Composition of Sugarcane Bagasse

Ref: Pandey et al 2000.

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 Sugarcane bagasse in Nanotechnology

Yield/Ton

Of SCB Size reduction to

nanodimensions
1 ton 280 kg

Packaging
matrices
High elastic modulus
Conductive High surface toSynthesis
volume ratio
polymers Lignocellulosic
Low density
PROPERTIES
composites
Thermostability
Nanofiller Bio degradability
agents
Bio-based Low Density 4

remediants
Ref: Gardner et al 2008, Klemm et al 2011.

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 OBJECTIVES

Isolation of chemically purified cellulose .

Extraction of cellulose nanofiber .

Incorporation of the extracted cellulose nanofiber in starch based

bioedible film.

Characterisation of the prepared film.

Effect of pretreatment?

-Destroy lignin shell protecting cellulose.

-Decrease crystallinity of cellulose.

- Must break this shell for enzyme to access substrate (sugar).

ISOLATION OF CHEMICALLY PURIFIED
CELLULOSE

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 Dewaxed in Delignification using an
As soxiet acidified sodium
Sugarcane apparatus
Received chloride solution of
with 2:1 75°C for 1 hand
(v/v)of repeated four to five
Sugarcane benzene and times until products
Bagasse methanol become white
(Grounded & 30 for 6 hours
mess screened) Next, product was
treated 2 wt% KOH
at 90°for 2 h

Filtered and At last, treated with

Chemically rinsed until 5 wt% KOH at 90°for
purified residues were 2h
cellulose neutral
EXTRACTION OF CELLULOSE
NANOCRYSTALS

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 Chemically Constant
64 wt% H2 So4
Purified stirring for 60 Centrifugation
soluion
Cellulose minutes at at 10,000 rpm
Pre heaing at 45°C
(CPC) 45°C for 15 mins

Centrifugation
Concentrated
Sonication for at 10,000 rpm Dialysis for 4-5 days
aqueous
10 mins in ice for 15 mins
suspensions of
bath
CNC,s
FTIR SPECTROSCOPIC MEASUREMENT

3383cm-1 Strain O-H

2901cm-1 Strain C-H

1637cm-1 O-H bond

of water
absorption
1371cm-1 C-O
stretching

1058cm-1 C-O and C-

H
stretching
 XRD ANALYSIS

The crystallinity index of the

CNC is found to be 65.7%

FIG: XRD graph of CNC

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 SEM MORPHOLOGICAL INVESTIGATION

(A) (B)

(C) (D)

FIG:Scanning electron micrographs of CNCs at different resolutions.

 PREPARATION OF FILM
Preparation of film
STARCH CELLULOSE
NANOFIBER

GLYCEROL DEIONISED
WATER

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 References
• Gardner, D. J., Oporto, G. S., Mills, R., & Samir, M. A. S. A. (2008). Adhesion and surface issues in cellulose and
nanocellulose. Journal of Adhesion Science and Technology, 22(5-6), 545-567.

• Klemm, D., Kramer, F., Moritz, S., Lindström, T., Ankerfors, M., Gray, D., & Dorris, A. (2011). Nanocelluloses: a
new family of nature‐based materials.Angewandte Chemie International Edition, 50(24), 5438-5466.

• Pandey, A., Soccol, C. R., Nigam, P., & Soccol, V. T. (2000). Biotechnological potential of agro-industrial
residues. I: sugarcane bagasse. Bioresource technology, 74(1), 69-80.

• Buragohain, M. R. I. D. U. L., & Sarma, H. P. (2012). A study on spatial distribution of arsenic in ground water
samples of Dhemaji district of Assam, India by using arc view GIS software. Sci. Rev. Chem. Commun, 2, 7-11.

• Mukherjee, A., Sengupta, M. K., Hossain, M. A., Ahamed, S., Das, B., Nayak, B., ... & Chakraborti, D. (2006).
Arsenic contamination in groundwater: a global perspective with emphasis on the Asian scenario. Journal of
Health, Population and Nutrition, 24(2), 142-163.

• Yu, X., Tong, S., Ge, M., Zuo, J., Cao, C., & Song, W. (2013). One-step synthesis of magnetic composites of
cellulose@ iron oxide nanoparticles for arsenic removal. Journal of Materials Chemistry A, 1(3), 959-965.

• Lee, H. V., Hamid, S. B. A., & Zain, S. K. (2014). Conversion of Lignocellulosic Biomass to Nanocellulose:
Structure and Chemical Process.The Scientific World Journal, 2014.

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 TEM ANALYSIS

FIG: Tem images of the film sample

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THANK YOU