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COMPATIBILITY TESTING
Out line
Collection and Preparation of Samples
Collecting Patient Samples
Donor Samples
Selection of Appropriate Donor Units
Testing of the donor sample
Testing of the patient sample
Cross-match
Summary of Pre-transfusion Testing
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COMPATIBILITY TESTING
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COMPATIBILITY TESTING
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COMPATIBILITY TESTING
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COMPATIBILITY TESTING
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COMPATIBILITY TESTING
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COMPATIBILITY TESTING
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COMPATIBILITY TESTING
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COMPATIBILITY TESTING
Donor Samples.
Donor testing samples must be taken when the full donor
unit is drawn. Depending on the method used for testing,
clotted sample, anti-coagulated samples, or both, are
obtained.
Donor information and medical history card, the pilot
samples for processing, and the collection bag must all
be labeled with the same unique number code before
starting the phlebotomy, and the numbers must be
verified again immediately after filling.
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COMPATIBILITY TESTING
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Compatibility Testing Protocols.
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Selection of Appropriate Donor Units.
Group O packed red blood cells can be safely used for all
patients, however, conservation of a limited supply of
group O blood should dictate its use for patients of other
AB types only in special circumstances.
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Selection of Appropriate Donor Units.
AB A, B, O
only one of the three should be used for a given patient
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Selection of Appropriate Donor Units.
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Selection of Appropriate Donor Units.
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Compatibility Testing Protocols.
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Testing of the donor sample
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Testing of the patient sample.
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Testing of the patient sample.
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Testing of the patient sample.
ABO Grouping.
Determination of the patient’s correct ABO group is the
most critical pre-transfusion serologic test.
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Testing of the patient sample.
Rh Grouping.
Rh grouping is performed using anti-D blood grouping
serum. Tube or slide tests should be performed
according to the manufacture’s directions for the
reagent, which may or may not include the use of a
suitable diluents control.
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Testing of the patient sample.
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Testing of the patient sample.
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Testing of the patient sample.
Antibody Screening.
The patient’s serum or plasma must be tested for
unexpected Abs.
The aim of the Ab screening test is to detect as many
clinically significant Abs as possible.
Clinically significant Abs refers to Abs that are reactive at
37°C or in the DAT or both and are known to have
caused a transfusion reaction or unacceptably short
survival of the transfused red blood cells.
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Testing of the patient sample.
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Testing of the patient sample.
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Testing of the patient sample.
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Testing of the patient sample.
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Testing of the patient sample.
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Cross-match
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Cross-match
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Cross-match
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Cross-match
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Cross-match
Antiglobulin Cross-match
The procedure begin in the same manner as the IS cross-
match, continues to 37°C incubation and finishes with
AHG test.
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SUMMARY
PRE TRANSFUSION TESTING
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PRE TRANSFUSION TESTING
Compatibility Testing
Each compatibility test is a unique experiment in which
an unknown (patient) serum and (donor) red cells are
tested for the detection of unexpected antibodies which
are directed against antigens found on the cells. Negative
results indicate compatibility. This is one of the most
important tests performed by a transfusion service.
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PRE TRANSFUSION TESTING
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PRE TRANSFUSION TESTING
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Patient red cell with –D Ag.
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If Rh –ve do
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