Documente Academic
Documente Profesional
Documente Cultură
Lead
Iron
Cadmium
Manganese
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Zinc
INTRODUCTION
• Tea is one of the most popular nonalcoholic beverages
in the world. As tea contains essential nutrients
consuming tea is beneficial for human health.
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• The main sources of heavy metals in plants are their
media, nutrients, agro inputs and soil. Other sources
may include pesticides and fertilizers.
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• In-vitro gastrointestinal extraction, also known as
bio accessibility, is important when assessing
chemical risk to humans.
Heavy Metals:
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MATERIALS & METHOD
INSTRUMENTS
• Flame Atomic Absorption Spectrometer (FAAS)
• Centrifuge
• Muffle furnace
• Microwave assisted digestion unit
• Mechanical shaker
REAGENTS
• Deionized water
• Standard Metal Solutions
• Enzymes and bile salts (Pepsin, Pancreatin, Bile extract)
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SAMPLE COLLECTION & PREPARATION
• Orthodox (Whole leaf )and CTC (Crush ,Tear, Curl)processed
tea samples will be collect from following major tea producing
areas according to Tea Market Update volume 12 ,No 2
Galle
Badulla
Matara
Kandy
• Organically grown Eliya
Nuwara tea sample will be collect as a control sample
• Soil andwater samples from cultivations will be collected random
Rathnapura
manner for heavy metal analysis
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Total Metal concentration Total Metal Concentration
in tea extract in bioaccessible fraction
Supernatant and
Untreated tea extract residue will be
will be wet ashed with separated and wet
5 mL of 50% HNO3 ashed with 5 mL of
50% HNO3
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STATISTICALStatistical
ANALYSIS Analysis
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TIME FRAME
Oct Nov Dec Jan Feb Mar Apr May Jun Jul Aug Sep
Literature survey
Experimental work
Thesis writing
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REFERENCES References
• Minekus, M., Alminger, M., Alvito, P.,et. al (2014) A standardised static in vitro digestion method
suitable for food - an international consensus. Food & Function. 5 (6) 113-1124.
• Falahi, E. and Hedaiati, R. (2013). Heavy metal content of black teas consumed in Iran. Food Additives
and Contaminants: Part B, 6(2), pp.123-126.
• Harmanescu, M., Alda, L., Bordean, D., Gogoasa, I. and Gergen, I. (2011). Heavy metals health risk
assessment for population via consumption of vegetables grown in old mining area; a case study:
Banat County, Romania. Chemistry Central Journal, 5(1), p.64.
• Ho, C., Lin, J. and Shahidi, F. (2009). Tea and tea products. Boca Raton: CRC Press.
• Seenivasan, S., Manikandan, N., Muraleedharan, N. and Selvasundaram, R. (2008). Heavy metal
content of black teas from south India. Food Control, 19(8), pp.746-749.
• Soylak, M., Tuzen, M., Souza, A., Korn, M. and Ferreira, S. (2007). Optimization of microwave assisted
digestion procedure for the determination of zinc, copper and nickel in tea samples employing flame
atomic absorption spectrometry. Journal of Hazardous Materials, 149(2), pp.264-268.
• J.M Laparra, D. Velez et.al (2003)Estimation of Arsenic Bioaccessibility in edible Seaweed by an invitro
digestion Method: J.Agric.Food che, pp51
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THANKYOU!!!
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