THE ROLE OF MICRO RNA IN DIAGNOSIS

AND
THERAPY OF BREAST CANCER

Wirsma Arif Harahap

Surgery Department – Andalas University
Globocan 2013

World Western Development Country

Biomolecular World of Breast Cancer

Undefined SNPs : 14%

50 % unexplained
Factors

1. Epigenetic( methylation, miRNA,

Histon asetilasi
2. Gene interaction
3. Protein interaction

Ferguss et al. 2014

Molecular Biology Changing
heritable and modifiable markers that regulate gene expression
without changing the underlying DNA sequence  genomic
stability and regulated gene expression
- Younger Age
- 60% BRCA1 Methylated
 WHAT IS MICRO RNA ?

- Discovered in 1993 by a group led by Ambros , Lee and Feinbaum.

- Small , non coding RNA molecule +/- 22 nucleotides
- Found in Plants, Animals, Human and Virus
- Human  more than 1000 miRNA
WHAT IS MICRO RNA ?
 WHAT IS MICRO RNA ?
• 1 miRNA can regulate more than 2
mRNA
• Target  mRNA  degradation /
inhibition
• miRNAs regulate 30–60% gen expression
ini human (Griffiths et al., 2008)
Role of miRNAs in Cancer
WHY MICRO RNA IS SO IMPORTANT ?
MICRO RNA REGULATION IN BREAST
CANCER
 TARGET MiR-10B
MiRTarBase
miR-10b

HOXD-10

RhoC

TIMP2 PI3KCA

Akt mTOR Proliferasi

Invasi dan metastase EM
T
Ekspresi miRNA-10b

0.00

Relative Expression of MiR-10b

-2.00

-4.00

-6.00

-8.00

-10.00

Ca Mammae Fibroadenoma
Group

Ekspresi relatif dari miR-10b antara jaringan kanker payudara (merah) dengan jaringan
fibro adenoma (hijau) sebagai kontrol (diagram box-plot dengan nilai median).

Perbedaan ekspresi miR-10b antara kanker payudara dan tumor jinak

Kelompok Rerata ΔCp SD p
miR-10b
Jaringan Kanker -5.61 1.35 0.000
Payudara
Jaringan Tumor Jinak -3.54 1.54
 Conclusion
• Given the role of miRNAs in cancer induction or prevention as well as
invasiveness or non-invasiveness, it seems that these micro molecules can
be used as biomarkers for early detection of breast cancer and progression.
• Release of miRNAs from the tumor to the blood circulation can be used as
diagnostic indicators.
• Levels of miRNAs’ expression can be considered in the monitoring of tumor
status.
THANK YOU