BOVINE OCULAR SQUAMOUS CELL

CARCINOMA.
(THIRD EYELID TUMOUR)

Submitted by: Submitted to:

Swaroop R Dr. B Kamal hasan

HVK 1661 Dept of VLD
Veterinary college ,Hassan
 ABOUT THE DISEASE
• Bovine ocular squamous cell carcinoma is more commonly referred to in the cattle industry as
“cancer eye”.
• Squamous cell carcinoma is by far the most common tumor afflicting the bovine eye, as well as the
most frequently diagnosed cancerous tumor in the bovine.
• This disease is of extreme economic importance to the cattle producer as it accounts for nearly
12% of carcass condemnation.
• The etiology (cause) of the disease is multifactorial. There is a predisposition towards cattle with
non-pigmented eyelids and conjunctiva (white faced cattle).
• Prolonged exposure to sunlight (ultraviolet light) also seems to be a driving force for the disease.
• Bovine papillomavirus and infectious bovine rhinotracheitis virus have been isolated from these
lesions, but their importance in the disease process is unknown.
• The tumors generally develop in cattle over the age of seven and rarely in cattle less than three
years. The most common areas the tumors are found are: the limbus (junction of the cornea and the
sclera), the third eyelid, and on the upper and lower eyelid margins.
 CASE SHEET

• Case no.:19131
Species:bovine
Breed:hf
Age:8 years
Sex:female
Observations:

Temp:101.8ºF
HR:72 beats/min
RR:36/min

CMM:Pink
Body weight:470 kg
 HISTORY AND DETAILED PHYSICAL
EXAMINATION
• Small mass growth on the right eye,lacrimation
seen from past 3 days ,normal feed and water
intake

• Cauliflower like mass growth on right eye near

medial canthus and pus discharge was seen
 DIAGNOSIS
• History
• Clinical signs
• Histopathological and
immunohistochemical processing and
grading
• Insitu hybridisation(Pappiloma virus)
• Giemsa staining
DIAGNOSTIC PROCEDURE
 GIEMSA STAINING TECHNIQUE

• Fix the impression smear in absolute methyalcohol for 2

min.This can be done by dipping the smear briefly in a
coplin jar containing methyl alcohol.
• Leave the smeat to dry
• Stain in a 1 in 10 dilution of Giemsa’s stain for 20 min(Add
1ml of stock Giemsa’s to 9ml of neutral buffered water)
• Wash the smear,air dry it and examine under oil immersion
objective.
MICROSCOPIC VIEW
 DIFFERNCE B/W BENIGN AND MALIGNANT
Benign
The morphology of the tumour is
approximately normal in relation to adjacent
tissues.
Minimal evidence of anaplasia few mitotic
figures
Doesnot extend behind basement membrane
Does not penetrate connective tissue capsule
Generative and necrotic changes within the
tumour are slight
There is no invasion of blood vessels
Malignant
The morphology of the tumour is abnormal in
relation to adjacent tissues.
Marked anaplasia abundant mitotic figures
Extends behind basement membrane
Penetrates through connective tissue sample
Degenerativeand necrotic changes within the
tumour are extensive
Invasion of the blood vessels and lymphatics
occurs
 OBSERVATION

• Impression smear stained with Giemsa revealed large number of RBC s and many
degenerative granulocytes
• Cluster of cells were found
• Few anaplastic cells were observed
 INFERENCE
•Malignant tumour
 TREATMENT

• Excision of the tumour by Surgical intervention

• Thermocautery
• Interleucin 2
• Radition therapy
• Immunotherapy
• Cryosurgery