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Introduction
Size Ranges of Biological
Material
• Cells: 100um – 10um
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Introduction
Nanobiotechnology is an emerging field
• cells discovered 1665
• electron microscope 1950s
• Watson and Crick discover DNA double helix 1953
• Mapping of Human Genome 2003
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WHAT IS NANOTECHNOLOGY?
Nanotechnology is
the manipulation of
matter at the
nanometer* scale
to create novel
structures, devices
and systems.
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Economic impact of
nanotechnology
Market Size Predictions
(within six years)
$340B/yr Materials
$300B/yr Electronics
$180B/yr Pharmaceuticals
$100B/yr Chemical manufacture
$ 70B/yr Aerospace
$ 20B/yr Tools
$ 30B/yr Improved healthcare
$ 45B/yr Sustainability
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Nanobiotechnology In Medical
Technologies
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Nanotechnology in Health
Care
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Nano-technology
Nanotechnology is a new field with many
possible uses, medicine being one of them.
Nanotech was first predicted by Nobel Laureate
Richard Feynman in 1959.
It’s anything to do with structures one to several
hundred nanometers long in at least one
direction. However, nanotechnology doesn’t
necessarily deal with nanometers, as it can be
expanded to include structures measured in
microns also.
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Present Major Medical
Applications
- Neurology
- Oncology
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Size of Markets
• Drugs & Drug Delivery $80 billion
• Blood Analysis $22 billion
• Heart Pacemakers & $16 billion
Other Implants
• Endoscopy & MIS Tools $5 billion
• Hearing Aids $4 billion
• DNA / Lab on Chip $1.2 billion
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Drivers in healthcare
– Ageing population
– Personalized health
– Earlier diagnosis
– Cost of healthcare
– Global travel (epidemics)
– Food safety
– Monitoring of individuals (glucose,
cholesterol)
– Ethics (eg stem cell research, animal
testing)
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Hot R&D Topics
• Lab on Chip
• Retinal Implants
• Non Invasive Blood Glucose
Monitoaring
• The ‘Electronic Rat’
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Timescales?
• Roadmaps based on
– current status of R&D
– Future vision
– Past experience
• Balance market drivers against
barriers
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Nano molecular diagnostics
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Lab-on-a-chip systems are miniaturized biosensors that,coupled with
portable instruments, promise to offer inexpensive, point of care
medical diagnosis. The technology is being tested for use in the
monitoring of HIV immune function.
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Lab-on-a-chip systems
Study of chromosomes by AFM
Application of nanopore technology for
molecular diagnostics
DNA protein and nano particle conjugates
Nanoparticle based colorimetric DNA
detection method
Nanobiosensors
Nanowirebiosensors etc
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Biobarcode assay for proteins
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Instrument for DNA
Detection
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Biosensors
These are the biosensors under development in world-class laboratories. Some
of these devices were developed in collaboration with PPP model.
HandHeld Submersible
model model
KinExA™
model BenchTop
model
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NANOPLEX™ biomarkers are robust, patented optical
detection labels that bring entirely new, much-needed
capabilities to clinical diagnostic and life science
measurements.
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Nanotechnology based biosensor prototype capable
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Cantilever arrays QD
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Applications
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Applications
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As diagnostics
• Biosensing
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Biological recognition elements
for sensors
Enzymes
-transformation of analyte into sensor detectable product
Antibody-antigens
-high affinity binding with tracer to generate a signal
DNA-ligand binding
Biomimetic sensors
-engineered molecules (single chain antibody fragment)
- supported lipid bilayers
-molecularly imprinted polymers
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Nanoarrays
Nanoarray with single-stranded DNA representing
thousands of different genes, each assigned to a
specific spots on a device.
NANOARR
AY
CHIP
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Protein arrays for diagnostics
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DNA-nanoparticle complexes
• DNA molecule
• DNA-nanoparticle
complexes based
on Au-thiol
binding
• Nanoparticle
labeling for
biochips
• Labeling of single
molecules
• Devices, e.g.
nanoelectronics.
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DNA-coated gold nanoparticles (NPs) system
that also uses larger magnetic microparticles (MMPs) to detect
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Nanotechnology and diagnostics
Dendrimers
Dendrimers, 1- to 10-nanometer
spherical polymers of uniform
molecular weight made
from branched monomers (Poly
imido amine), are proving
particularly adept at providing
multifunctional modularity.
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Nanotechnology in observing
disease processes involving fibrils
using AFM
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Drug encapsulation and delivery
with nanoparticles
vehicles for delivery
• coated solid particles
• vesicles
• liposomes
• micelles
• polymers
• solid lipid nanoparticles
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Nanoparticle as Delivery System for Drugs
or Genes for Tissue and Cell
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Intracellular trafficking of nanoparticles
Nanoparticles eventually act as intracellular
reservoirs for sustained release of
encapsulated therapeutic agent.
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Tissue targeting of nanoparticles
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Cell microencapsulation in polymer
matrix
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Enhanced Permeability and Retention
(EPR) effect)
Nanoparticle entry and accumulation in tumors
• PEGylated particles, in the desired size range, leaks out of the
microvasculature and accumulates into the tumor site.
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Nanotechnology and cancer
therapy
Silica coated lipid micelles
Silica-coated lipid
micelles containing
LH-RH as a targeting
agent have been
used to deliver iron
oxide particles to LH-
RH receptor- positive
cancer cells
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Micellar Nano-particles in cancer therapy
Lymphatic delivery
Tumor conc. of 99mTc labelled
Etoposide and EPM 1, 6, 24
hours after S.C.Inj.
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10
8
6
4
2
0
Time (h)
• Phase separation
process during
cooling in solid
lipid nanoparticle
(SLN) production
leading to a drug
enriched shell
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Tumor accumulation Studies with
Etoposide nanoparticles (ETPL)
• Tumor accumulation of 99mTc-Etoposide and 99mTc-
ETPL in Dalton’s lymphoma tumor bearing mice
• Radioactivity measured 1h, 6h and 24h after
subcutaneous administration
Ref: Reddy & Murthy:AAPS Journal, 6(3), Article 23, 2004
2 a : ET
b : ETPL
1 1 : 1h
2 : 6h
0
3 : 24h
1a 1b 2a 2b 3a 3b
Time (h)
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