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LOCK AND KEY

HYPOTHESIS

Presented by
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 Class & Board
Ö Ê

  



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Essential Questions
Ö How do biochemical reactions proceed so
fast?
·nit Questions
Ö How does enzyme function ?
Ö Where does enzyme go after performing its
function?
Content Questions
Ö What is lock and key hypothesis of
enzyme functioning?
Ö How are enzyme substrate
complexes formed?
Ö How is product released from its
enzyme complex?
O
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Ö ecture cum Demonstration Method
 Lock and Key
Hypothesis
Ö When in solution molecules of enzyme
and substrate move about
Ö An enzyme molecule collides with its
substrate molecule and the substrate
binds to the enzymes active site
Ö Together they form an enzyme-substrate
complex

cont.
ëEnzyme ²substrate complex is formed
because they Have complementary
shapes
ëSpecific substrate molecules are
bound to a specific region of the
enzyme called its active or
catalytic site
ëThe active site is formed by a
number of amino acid residues

cont
 whose side chains have two
principal roles to play-:

Attract and orient substrate in a

specific manner within The site.

articipate in the formation of

temporary bonds with the ubstrate
molecule which tri er the
catalytic chan e.
 The lock and key
mechanism
Active Site

Substrate
Enzyme
The weak bonds
between the substrate
and the enzyme
require energy, which
Enzyme-substrate is provided by the
complex bonds in the substrate.
Catabolism
Ö Water then moves between the bonds in the
substrate to break them«
The Product

The product then

leaves the active
site as the bonds
are sufficiently
weakened

The enzyme returns to its

original form ready to react
with the substrate again
‘

Ö What happens to the
chapatti once it enters
our body ? Explain in terms of ock and key
mechanism of enzyme action.
2

Ö iology ±Class XI (NCERT)
Ö Modern¶s abc of iology (by . Arora
&A.K Sabharwal)
Ö www.rsfoxlander.edu
Ö www.campbelmtu.edu
Ö www.co perativebinding_files
O

 Active Site

Substrate
Enzyme

Enzyme-substrate
complex