NOTE: Dr. Chiaia has posted pretty good images in a pdf file on the intranet.

They are included here; virtual slides taken during class are used to
supplement
 UW 076 – Pig liver
Pig liver has lobules
clearly defined by lobules
connective tissue –
unlike human liver
 Central vein

Classic lobule (central vein in

middle, portal triads at corners)
Simple squamous
lining of central vein
 Also has lymph
vessels, but
usually collapsed
in slides

Bile duct is
most
conspicuous
of the triad –
cuboidal
cells around
lumen

Hepatic cords

Hepatic cords
-composed of hepatocytes (parenchymal
cells) NOTE: central veins
converge and
-arranged in “single file”
eventually drain into
-radiate away from central vein IVC via hepatic veins
(p. 54)
 Hepatic
MCO0083 (20x)
cord
Probably WBC (said Dr. Crissman)

Space of
Disse
(between
sinusoid and
hepatocyte)

sinusoid

Bile canaliculi (where?) - “small openings” or “perforations” between adjacent

hepatocytes in the cords; run at right angles to the hepatic cords; will appear as a
small dark spot or squiggle on the line about midway between the two sinusoids on
each side of the cord. –p. 55
Hepatic artery
Hepatic vein
Bile duct
 Liver, Kidney, Uterus - UW2152 (low mag)

uterus

liver

kidney
 UTN002 – Mammal Liver, Kupffer’s
Kupffer cells - with ingested blue
stain particles
Pancreas - MCO0086 (low mag)
 MCW082 – Pancreas, H&E (high mag)

Islet of
Langerhans

Acinar cells
Centroacinar cells -nuclei and RER are in basal region – stain
-lighter staining than acinar cells basophilic
-apical region has secretory granules; stain
-secrete bicarbonate (buffer) acidophilic
-form initial portion of intercalated -secretions are serous
 centroacinar

Acinar
Intralobular ducts
coalesce into an
interlobular duct
-Intercalated duct
associated with
centroacinar cells
Interlobular artery (probably won’t have to
identify artery)
“Delicate reticular fibers form a branching network that supports the central veins,
sinusoids and bile ducts. Look for the silver-stained, interconnecting black fibers of type
III collagen weaving around the hepatocytes to give delicate support without blocking
movement of fluids across the space of Disse.”
p. 55
-Light staining
-are the endocrine
portion of pancreas
Islets of Langerhans
-light staining
MCO 0085 – Gallbladder (low mag)
EPI – Epithelium (simple columnar)
ME – Muscularis externa
PMCT – perimuscular connective tissue
SS EPI – Simple squamous epithelium
(serosa)
 Gallbladder layers

Simple squamous
epithelium
Perimuscular CT

muscularis

Lamina propria –
“contains some
lymphocytes,
fibroblasts, and
capillaries” p. 56

Epithelium (can’t distinguish

brush vs. clear cells at this
level)