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Cell Communication
Biologists
Have discovered some universal mechanisms of cellular regulation
Figure 11.1
Copyright 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Concept 11.1: External signals are converted into responses within the cell
Receptor a
factor
2 Mating. Binding
of the factors to receptors induces changes in the cells that lead to their fusion.
3 New a/ cell.
Figure 11.2
The nucleus of the fused cell includes all the genes from the a and a cells.
a/
Figure 11.3 (a) Cell junctions. Both animals and plants have cell junctions that allow molecules
to pass readily between adjacent cells without crossing plasma membranes.
Figure 11.3 (b) Cell-cell recognition. Two cells in an animal may communicate by interaction
between molecules protruding from their surfaces.
Secretory vesicle
Local regulator diffuses through extracellular fluid (a) Paracrine signaling. A secreting cell acts on nearby target cells by discharging molecules of a local regulator (a growth factor, for example) into the extracellular fluid.
Target cell is stimulated (b) Synaptic signaling. A nerve cell releases neurotransmitter molecules into a synapse, stimulating the target cell.
Figure 11.4 A B
In long-distance signaling
Both plants and animals use hormones
Long-distance signaling Endocrine cell Blood vessel
Target cell
Figure 11.4
(c) Hormonal signaling. Specialized endocrine cells secrete hormones into body fluids, often the blood. Hormones may reach virtually all C body cells.
Sutherland suggested that cells receiving signals went through three processes
Reception Transduction Response
Response
Concept 11.2: Reception: A signal molecule binds to a receptor protein, causing it to change shape
Steroid hormones
Bind to intracellular receptors
Hormone EXTRACELLULAR (testosterone) FLUID
1 The steroid
hormone testosterone passes through the plasma membrane.
Receptor protein
2 Testosterone binds
to a receptor protein in the cytoplasm, activating it.
DNA mRNA
3 The hormonereceptor complex enters the nucleus and binds to specific genes. 4 The bound protein
stimulates the transcription of the gene into mRNA.
NUCLEUS
New protein
Figure 11.6
CYTOPLASM
Receptors in the Plasma Membrane There are three main types of membrane receptors
G-protein-linked Tyrosine kinases Ion channel
G-protein-linked receptors
Signal-binding site
G-protein-linked Receptor
Plasma Membrane
Activated Receptor
Signal molecule
Inctivate enzyme
GDP
CYTOPLASM
G-protein (inactive)
Enzyme
GDP
GTP
Activated enzyme
GTP
GDP
Pi
Figure 11.7
Cellular response
Signal-binding sitea
Signal molecule
Tyrosines
Tyr Tyr
CYTOPLASM
Dimer
ATP
6 ADP
P Tyr
Figure 11.7
Gate closed
Ions
Plasma Membrane
Gate open
Cellular response
Gate close
Figure 11.7
Copyright 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Concept 11.3: Transduction: Cascades of molecular interactions relay signals from receptors to target molecules in the cell Multistep pathways
Can amplify a signal Provide more opportunities for coordination and regulation
In this process
A series of protein kinases add a phosphate to the next one in line, activating it Phosphatase enzymes then remove the phosphates
A phosphorylation cascade
Signal molecule Receptor
3 Active protein kinase 2 then catalyzes the phosphorylation (and activation) of protein kinase 3.
Active protein kinase 3 P
ADP
5 Enzymes called protein phosphatases (PP) catalyze the removal of the phosphate groups from the proteins, making them inactive and available for reuse.
Pi
PP Inactive protein
4 Finally, active protein kinase 3 phosphorylates a protein (pink) that brings about the cells response to the signal.
P Active protein Cellular response
ATP ADP Pi PP
Figure 11.8
Copyright 2005 Pearson Education, Inc. publishing as Benjamin Cummings
NH2 N N O N
O O
O O
N O
O P O P O P O Ch2 O
HO P O CH2
OH OH ATP
OH OH AMP
Figure 11.9
Many G-proteins
Trigger the formation of cAMP, which then acts as a second messenger in cellular pathways
First messenger (signal molecule such as epinephrine) G protein Adenylyl cyclase
G-protein-linked receptor
Figure 11.10
Copyright 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Cellular responses
Calcium ions and Inositol Triphosphate (IP3) Calcium, when released into the cytosol of a cell
Acts as a second messenger in many different pathways
Nucleus CYTOSOL
ATP
Ca2+ pump
Ca2+ pump
Figure 11.11
Key
High [Ca2+]
Low [Ca2+]
Other second messengers such as inositol triphosphate and diacylglycerol Can trigger an increase in calcium in the cytosol
2 Phospholipase C cleaves a plasma membrane phospholipid called PIP2 into DAG and IP3.
G-protein-linked receptor
Phospholipase C
PIP2
IP3 (second messenger)
Ca
2+
Cellular response
Figure 11.12
5 Calcium ions flow out of the ER (down their concentration gradient), raising the Ca2+ level in the cytosol.
6 The calcium ions activate the next protein in one or more signaling pathways.
Concept 11.4: Response: Cell signaling leads to regulation of cytoplasmic activities or transcription
Transduction Inactive G protein Active G protein (102 molecules) Inactive adenylyl cyclase Active adenylyl cyclase (102) ATP Cyclic AMP (104) Inactive protein kinase A Active protein kinase A (104) Inactive phosphorylase kinase Active phosphorylase kinase (105) Inactive glycogen phosphorylase Active glycogen phosphorylase (106) Response Glycogen Glucose-1-phosphate (108 molecules)
Figure 11.13
Copyright 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Other pathways
Regulate genes by activating transcription factors that turn genes on or off
Growth factor Receptor
Reception
CYTOPLASM
Response
Gene
Figure 11.14
NUCLEUS
mRNA
Response 1
Response Response 2 3
Figure 11.15
Scaffolding protein
Figure 11.16
Copyright 2005 Pearson Education, Inc. publishing as Benjamin Cummings