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Strategical Solution for a problem

Bioremediation Reddy
11

M.Santhosh

10MSM0016

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Initial Survey for wastes

Location Identified Contaminants: Railway Track, Outside Gate 2 Paper(Low GSM), Plastics, PET bottles, Carton(High GSM), Organic waste food, Leather Debris, Hair Debris, Thick PVC pipes, Iron pieces. Mostly solid. Organic waste = semi solid. Cellulose, Polyethylene, Poly ethylene terapthalate (PET), Poly Vinyl Chloride(PVC).
22

Physical Properties: Chemical:

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What if they are

???

33

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Environmental effects
Paper Soil Foul Fine

attracts microbial activity

Pollution due to Plastics, PET. smell. hair debris Turbulence. spread case death of microbes
44

Infertility Disease Extreme

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Solution?? Yes, Bioremediation

55

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Contaminants
Cellulose Polyethylene Polyvinyl Hair Fe

Chloride

debris debris

pieces

Leather

66

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Cellulose ; detection
Visual

method method technique

Check for paper.

Biological

CBD + fluorescent + cellulase.

Analytical

GC-MS.

77

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CELLULOSE ; BIODEGRADATION
Trichoderma viridae -1,4 glucanase

-glucosidase

88

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Poly ethylene ; detection

Visual

detection means methods

Physical

X-Ray diffraction detects nanograms

Analytical
GC MS

99

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Polyethylene ; biodegradation
Polyethylene
Lad A Mono oxygenase

Primary Alcohol Aldehyde Fatty acid

Geobacillus thermodenitr ificans

Alcohol dehydrogenase Aldehyde dehydrogenase

1010

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Pvc ; biodegradation
Polyvinyl Chloride
Vinyl chloride reductive dehydrogenase

Ethylene

Dehalococc oides

Succinyl CoA
1111

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Hair debris
Contains Use

keratin.

of keratinolytic fungi

Trychophyton ajelloi keratinophyllum

Chrysosporium

1212

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Leather debris
Mostly Highly Mostly

consists of Tannins. proteinaceous, degraded by fungi,

Aspergillus Penicillium

Bacteria
Bacillus

subtilis Alkaline protease(June

2006)
1313

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Iron pieces
Recyclable Re-usable Collected
Manual

picking

Magnets

Processed

1414

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Strategy:
Ex-

situ
Manual

Separation
picking
Magnets

Pre-treatment
Decrease

surface area

Detection Processing Analysis

1515

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Process

Analysis

Analy sis
1616

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Stirred tank bioreactor

1717

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conclusion
Leather Hair

96 hrs.

debris 3 months.

1818

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1919