IAIAN|COlAOU

81A|N
|N1kODUC1|ON 1O IAI 81A|N
Cooslstoocy oou rolloblllty oro too stololo coroor
stooos oI cytololcol lotorrototloo.
8cbtlo coll ooorooco coostootly bolo coorou
oou ossossou ucrlo scrooolo oou cytololst roly
ooovlly oo too gcollty oou too ooorooco oI too
stolo.
Ocollty cootrol oou Irogcoot ooltorlo oro
oouotory bct too rlocllos lovolvos oro too soo os
tooso lo too olstololcol stolo
t ls too colvorsol stolo Ior cytololcol roorotloo.
uorrls's uoootoyllo ls too otlc occloor stolo oou
too coblootloo oI OG oou |A 50 lvo too scbtlo
rooo oI rooo, blco oou lo| ocos to too coll
cytolos.
Vost loborotorlos cso coorclol stolos oou ooco wlll
coosluor toolr oulIlcotloos oI too orllool tocoolgco to
bo too otlc (Drl|vor 8 ooo}. Joo oou rosclt,
oowovor, sooclu rotolo too troosoroot gcollty oI too
cytoloslc stolo. (oo ovoroll sollu oooooocs
ooorooco ls oot too woy looolcolooc sow ols colls},
oou too occloor corootlo sooclu bo ooslly
ulstloclsoou.
Jyos oI stololo otoous
.-
l.lrorosslvo stolo
2.korosslvo stolo
lrorosslvo otoou
o tols rorosslvo otoou too occlocs ls stoloou wlto
oootoyllo to too lotooslty uoslrou.
Joo blcolo ooot Iollowlo too oootoyllo, sots
too occloor uyo lo loco.
Joo cytolos ls boroly tlotou.
Aoolc oyuroluo, lltolc corboooto, oou scott
to wotor scbstltcto oro too ost coooly csou
blcolo ooots.
Jo wotor oy sorvo os o blcolo ooot lI too u ls
oloor tooo 8.
lrorosslvo lrocoucros
|lotloo lo 80% lsorooool Ior 30 loctos
|lotloo lo 95% lsorooool Ior 30 loctos
uyurotloo lo 80% otoyl olcoool(lI olcoool Ilou}, 80%,
70%, 50%, otoyl olcoool l0 uls ooco.
Glvo l2 uls oI ulstlllou wotor.
uorrls's ooootoyllo 2-= loctos
Dlstlllou wotor 5 uls
0.5% ooolc oyuroluo Ior 30 socoous.
Woso wlto rcoolo to wotor 5 loctos.
Dlstlllou wotor l0 uls..
50%, 70%, 80%, 95% otooool l0 uls ooco
OG Ior 2 loctos
95 % otooool Ior 3 uls.
|A 3 =- loctos.
95% otooool 3uls.
Absolcto otooool 5 uls.
Ab. |tooool 3 uls.
Coblootloo oI olcoool oou ylooo (l.l}- 5 uls
xylooo 5 uls
Vocot lo Dlx.
korosslvo Votoou
uslo too rorosslvo otoou , ooo uollborotoly ovor
stolos too occlocs wlto o ooooclulIlou oootoyllo.
Joo ocoss stolo ls roovou wlto o ullctou uCl
solctloo.
Joo uocolorlzlo oclu ls tooo roovou by o boto or
rcoolo to wotor.
Jllo lo too oclu boto ls ossootlol Ior too Ilool
ooorooco oI too occloor ottoro.
Voro oItoo tooo oot, oyocorooslo rotoor
tooo oyorcorooslo ls tols rosclt.
Joo cytolos os woll os too occlocs ls
uocolocrlzou by too ullctou uCL.
I too oclu boto ls loouogcoto too cootrost
botwooo too corootlo oou orocorootlo ls
loss oou cto|o oI too cocotorstolos ls lossooou.
Papanicolaou formula
Harris`s hematoxylin:-
Hematoxylin 5g
Ethanol 50ml
Potassium alum 100g
Distilled water (50C) 1000ml
Mercuric oxide 2-5g
Glacial acetic acid 40ml
Orange G 6:-
Orange G (10 aqueous) 50ml
Alcohol 950ml
Phosphotungstic acid 0-15g
50
0.04 M light green SF 10ml
0.3 M eosin Y 20ml
Phosphotungstic acid 2g
Alcohol 750ml
Methanol 250ml
Glacial acetic acid 20ml
Filter all stains beIore use
looolcolooc stololo otoou
l. o. koovo olyotoylooo lycol Ilotlvo lo 50 % olcoool, 2 lo.
b. uyuroto lo 95% olcoool, 2 lo, oou 70% olcoool, 2 lo.
2. kloso lo wotor, l lo.
3. 8tolo lo uorrls's oootoyllo, 5 lo.
=. kloso lo wotor, 2 lo.
5. DlIorootloto lo 0.5 % ogcoocs oyurocolorlc oclu, l0 socoous
oro.
. kloso lo wotor, 2 lo.
7. 'lco' lo 8cott's to wotor scbstltcto, 2 lo.
8. kloso lo wotor, 2lo.
Coot.
9. Dooyuroto, 70% olcoool Ior 2lo.
l0. Dooyuroto, 95% olcoool Ior 2lo.
ll. Dooyuroto, 95% olcoool Ior 2lo.
l2. 8tolo lo OG , 2 lo.
l3. kloso lo 95% olcoool, 2 lo.
l=. kloso lo 95% olcoool, 2 lo.
l5. 8tolo lo |A 50, 3 lo.
l. kloso lo 95% olcoool, l lo.
kosclts .-
Joo occlol sooclu ooor blco,bloc|
Cytolos blco,rooo
korotlolzlo colls lo|,orooo
Noto .- Coooo stolos Irogcootly
Joo stololo tlos coo bo ou|cstou to sclt orsoool
roIorooco Ior o uor|or or olor stolo.
Altorootlvos to 8cott's to wotor scbstltcto loclcuo
0.l % oI ooolc wotor or o woo| ogcoocs
solctloo oI lltolc corboooto.
|octors oIIoctlo too lAl stolo .-
Jyo oI Ilotlvo csou
Jyo oI ooootoyllo Iorclo soloctou
|orclos oI too cocotor stolos
Looto oI too stololo tlo
Ncbor oI too slluos stolo ooco ortlcclor uyo.
u oou coolcol cootoot oou to wotor csou.
Joorotcro oI too wotor
u oI too solctloo oou too uyo
Ao oI too uyo
lrosooco oI too uyo ortlclos lo coIlltorou solctloo
losslblo cootolootloo oI uooyurotlo solctloo
o cooslstoocy oI stololo tocoolgcos
Jyo oI stolo csou
Ocollty oI too roorotloo oI too coll solo by too
cllolcloo.
u oI too socloo
lrosooco or obsooco oI too loIlootory coll coooos.