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Aims
1. 2. 3. 4. 5. 6.
Emphasise the wonders of water and some science of water. De-mystify some parts of water chemistry and show patterns BUT.some bits will confuse even more! Explain the meaning and Relationship of common water quality parameters Discuss the measurement and relation of water parameters to fish health Set a platform for future study
Ian Millichip
Overview
Water. The Magic Chemical Directions Measurements and Meaning pH/Acidity/Basicity/Alkalinity pH Buffers Hardness Redox Methylene blue Nitrogen Cycle and filtration Water Treatment/Chlorine/chloramine Magnesium/Calcium complex convolution
Ian Millichip
Ian Millichip
If the pH is acidic does all ammonia exist as ammonium? NO * Can pH be low and Alkalinity be high? YES Can you have Soft Alkaline water? YES If you measure pH, can you know how much acid is in the aquarium? NO *
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Water Is
Oxidane An Anomoly
Water Liquid at Room Temp Hydrogen Sulphide toxic gas Selenium Hydride toxic flammable gas
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Water Is
Magical Great Solvent (but not for water) Specific heat capacity (High) Heat of Vaporization ~ Humidity Air Miscibility and condensation / From Ice to Gas Density of Water vs Ice (4 C) Low Compressibility Cohesion and adhesion
Surface tension (cohesive strength) Capillary action (adhesive strength)
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Water Is
Life
Cradle of Life Vital Biochemical
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Change
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Change
HIGH ENERGY
In Short..
Chemistry is A downhill slippery and messy slope with Getting to Equilibrium and
Having Minimum Energy AND Giving out the Most Useable Energy
LOW ENERGY
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Ian Millichip
Starting With
R.O. Unit
?? What is in it ??
Fish Tank
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Ian Millichip
Conditioners
Dechlorination
Sodium thiosulphate (produces ammonia from Chloramines)
Sodium hydrosulphite (eg Seachem Prime as a complex) Sodium hydroxymethylsulfinate (eg AquaSafe) Sodium hydroxymethanesulfonate (eg Amquel)
Other Additives
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Chloramines
To RO Unit
Ammonia (UIA)
Activated Carbon
(Low Peroxide No.)
RO Membrane
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pH > 7.5
Acids/Bases et al
Acid
Donate a hydrogen ion to solution (classical understanding); OR Accepts an electron pair (eg Aluminium Chloride)
Base
Accepts a hyrdrogen ion; OR Donates an electron pair
pH Buffer
Resists changes of pH on addition of a small amount of acid or base Often mix of a weak acid or weak base and the salt of a weak acid or weak base
Alkalinity
Type of buffering. MEASURE..solutions ability to neutalise an acid. Linked in Aquaria to Carbonate buffering (and hardness)
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Hardness
Classically.Hard to form a lather Temporary Hardness
Easily removed by boiling heating decomposes soluble Bi-carbonates (Hydrogen Carbonates) to insoluble Carbonates
Permanent Hardness
Removed
by
Hardness
Contributes or Affects
General stability pH pH Buffering Alkalinity RedOx and RedOx Balance Ionic balance/conductivity/TDS Diffusion/Osmosis Nutrient Uptake Supply of vital minerals: calcium & magnesium
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200-500 S /cm
50-100 S/cm
M alaw i C ichlids Indian glass fish Firem outh & C onvict C ichlid (and som e other C ichlosom a type cichlids) P laty & Sw ordtail M ost R ainbow Fish W hite cloud m ountain m innow Am erican Flag Fish (Jordanella floridae) S catophagus argus C elebes R ainbow Fish M olly & G uppy G oldfish Tanganyikan C ichlid
100-200 S/cm
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RedOx
RedOx = Reduction and Oxidation RedOx Balance is vitally important. Complex RedOx Potential (ORP)
Potential
to Donate Electrons (Reducing Environment) Potential to Accept Electrons (Oxidising Environment) Is just a measure (and askwhat is measured?)
Reducing Environment is ideal for fish health Oxidising Environment is good for sterilising RedOx balance can get run-down with time.
Regular Partial Water changes; good aeration Having a good buffer Monitoring and maintain pH, Alkalinity, Hardness help.
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RedOx
Vital Consideration
The basis of life existing pivots around RedOx Potentials General Health and Water Quality In Aquaria(for examples).
Exchange across Gills Interaction at mucous layer Dropsy/Kidney Function/ Exploitation of substrate buffering Old-Water syndrome Biological Filtration Decay upsets RedOx Balance Treatment of Disease Treatment of certain poisonings
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Methylene Blue
RedOx Agent
Photosensitiser
Stain for microscopy and clinical diagnosis Redox Indicator ** Methaemoglobinaemia / Cyanide Poisoning / Carbon Monoxide Poisoning **
Hepatitis C/ Kaposi's sarcoma/ inactivates Staphylococcus aureus Anti-Malarial Induces Cancer Cell Apoptosis ** Protects against Mustard Alkylating Agent Neurotoxicity ** Mono Amine Oxidase Inhibitor used to make anti-psychotic drugs **
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Test Indicators
A pH Indicator (Thymol Blue) Nitrte/Nitrate Test
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Best Before Date; Store as directed Keep ALL documentation Use a syringe to measure water sample. Do not cross-use (cross-contaminate) test phials
Be aware that test kits are not 100% accurate, and are 100% specific. Take special note if aquarium water is tinted If using drop-by-drop count, let drops simply drop
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For probe-based equipment Make sure calibration fluids are proper standards Rinse in old calibration fluids before calibrating. Clean using distilled vinegar and store in RO/DI water Have water stirred whilst testing Treat as a Delicate Instrument Precision versus Accuracy?
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Lobe -LUMO Lewis acids Lobe -HOMO Lewis bases Onium Ion Lewis acids HOMO Lewis bases s-LUMO Lewis acids Lobe -HOMO Lewis bases Lobe -HOMO Lewis bases Lobe -LUMO Lewis acids Heavy Metal Lewis acids
) ); Ferrous Ion
Ferric (Iron III) chloride (FeCl 3), 3+ Ferric Ion (Fe ); Cupric Ion (Cu (Fe 2+ ); Lead II ion (Pb 2+ ); Silver ion (Ag +) Hydride Ion (H -) Hydrogen (H 2) + Hydrogen Ion or Proton (H ) Hydroxide ion (OH )
2+ Magnesium Ion (Mg ) Mercury (Hg); Iron metal (Fe) Methane (CH 3)
ACID ACID BASE BASE ACID BASE ACID ACID BASE BASE ACID ACID BASE
s-HOMO Lewis bases s-HOMO Lewis bases Proton Lewis acid Lobe -HOMO Lewis bases s-LUMO Lewis acids Heavy Metal Lewi s acids Lobe -HOMO Lewis bases Lobe -HOMO Lewis bases Onium Ion Lewis acids s-LUMO Lewis acids Lobe -HOMO Lewis bases
Nitrite ion [NO 2]Oxonium ion [OH 3]+ Sodium ion (Na +) Water (H 2O)
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Acids in Water
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pH (Strong Acid)
Hyrogen Chloride (HCl) In water = Hydrochloric Acid
(1 mole HCl = 36.5g)
Grams HCl 3700 1850 370 37 3.7 ~ pH 1.00 1.30 2.00 3.00 4.00
0.37
0.037 0.0037 0.00037 0.000037
5.00
5.96 6.70 6.96 7.00 7.00 7.00 7.00
1000 litres
0.0000037 0.00000037 0
Ian Millichip
pH (Weak Acid)
Acetic Acid (HAc)
(1 mole HAc= 60g) pKa = 4.75
Grams HAc 6000 3000 600 60 ~ pH 2.88 3.03 3.38 3.88
4.38 4.88
5.37 5.85 6.29 6.63 6.85 6.95 7.00
0.6
0.06 0.006 0.0006 0.00006
1000 litres
0.000006 0.0000006 0
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pK a = Log 10 ( K a )
pH = Log 10 ( {HA}KpKa1 a)
pKa2 pKa3
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Nitrogenous Compounds
Surplus amino acids need to be excreteddepends upon the bodys water demands and supply.
Reptiles > urates/uric acid with very little water Marine Fish > dimethyl amine or as urea in blood In humans > urea dissolved in plenty of water Freshwater fish > direct movement of ammonia out of gills (mainly by passive diffusion) Diffusion of Ammonia out of fish depends on ammonia in outside water and pH.
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Nitrogenous Compounds
In the Aquarium
Amino Acids / Waste / Dead fish / uneaten food
Ammonification [RAPID]
Ammonia (NH3)
0.06 mg/L
Ammonia
Filter Media
Inert
{all are mechanical} Inert Rocks/Pebbles
Sponge Wool Ceramic or special glass chips Membranes {eg Reverse Osmosis; removes selected molecules}
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Plants {lower nitrates} Activated Carbon {catalyst; remove certain chemicals} Clay {softens; remove chemicals; add minerals} Calcium Carbonate chips {Redox & Alkalinity Buffer} ## Aluminium oxide or Ferric oxide base. {Phosphate} ## Denitrifying Modified Beds {Nitrate Removal}
# Zeolites/Molecular Sieves {remove ammonia etc; removes and exchanges various ions}
# = Care - nutrient hazard ## = Extra Care - toxic hazard
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Ian Millichip
A Complex Convolution
Incorporation of Magnesium and Strontium into Corals Ion-Pair interactions changing solubilities and pH Buffering pH affecting solubilities
In Water
Biological Uses..
RedOx, Hardness (GH), Conductivity, Ionic Strength, ion-pair effects, SOLUBILITY, marine pH Buffering, (plus often associated in molecular form with Acid/Bases/Alkalinity)
Key Catalyst, Messenger Nerve impulses Nucleotide association (polyphosphates) eg DNA, RNA, ATP. Chlorophyll (a Magnesium porphyrin) And more Diabetes, migraines, osteoporosis, neurological (maybe depression). Alcohol lowers magnesium. Unbalanced protein intake inhibits magnesium absorption.. Coffee, Tea, Spices and Nuts, Green Veg., FISH FOOD !! Key Secondary Messenger. Nerve impulses Blood Clotting Cell Division Fertilisation Muscle action Immune Responses Regulator of Osmotic Stress. Bone Tetany (seen in cows), Rickets, etc Overfloading can cause excitotoxicity (eg after a stroke) Nuts, Milk, some green veg, shells.
Health (deficiency)
Sources (examples)
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C a2 + pp m
C a2 + pp m
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Summary
1. 2. 1.
No amount of Knowldege can make up for poor Water Management. Water Chemistry is complex
Standard Text Book chemistry does not necessarily apply You may not want to know it. But your Fish DO.
Water Chemistry is not an option. !! Do not mess with Water Chemistry unless one understands the implications Understanding the complexity may help explain unexplained Know the requirements of your fish Linking the Science to the Experience will help develop Aquatic Husbandry Many apologies for use of technical or scientific terminology.it is not jargon and allows communication with a wider audience
Ian Millichip
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