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Dr.T.V.Rao MD
Dr.T.V.Rao MD
Scientific Beginning
It was first described by Adolf Weil in 1886 when he reported an "acute infectious disease with enlargement of spleen, jaundice and nephritis". Leptospira was first observed in 1907 from a post mortem renal tissue slice.
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Leptospirosis - Zoonosis
Leptospirosis is an acute arthropod-zoonotic infection of worldwide significance caused by spirochete Leptospira interregna's which has 23 serogroups and >200 serovars. Various factors influencing the animal activity, suitability of the environment for the survival of the organism and behavioural and occupational habits of human beings can be the determinants of incidence and prevalence of the disease.
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What is leptospirosis?
Leptospirosis, also known as canicola fever, haemorrhagic jaundice, infectious jaundice, mud fever, spirochetal jaundice, swamp fever, swineherd's disease, caver's flu or sewerman's flu, is a bacterial infection resulting from exposure to the Leptospira interrogans bacterium.
Dr.T.V.Rao MD
Dr.T.V.Rao MD
Leptospirosis
There is an acute form of human infection known as Weil's disease, where the patient suffers from jaundice, though this term is often (incorrectly) used to describe any case of infection..
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Leptospirosis 2011
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Synonyms
Mud / Swamp fever Japanese 7 day fever Rice Field Fever Spirochete Jaundice
Canicola Fever
Leptospiral Jaundice
Autumn Fever
Swineherds Disease
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Reservoirs
Wild and domestic animals rodents, livestock (cattle, horses, sheep, goats, swine), canines, and wild mammals are the reservoir for leptospirosis. Many animals have prolonged leptospiruria without suffering from the disease themselves.
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Classification:
Phylum: Spirochaetes Class: Spirochaetes Order: Spirochaetales Species: Leptospira Family: Leptospiraceae
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Morphology
The Leptospira appear tightly coiled thin flexible Spirochetes 5 15 microns long. Fine spiral of 0.1 0.2 microns One end appears bent forms a hook. Actively motile Seen best with dark field Microscopy.
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Modes of Transmission
1. Direct contact with urine or tissue of infected animal Through skin abrasions, intact mucus membrane
2. Indirect contact
Broken skin with infected soil, water or vegetation Ingestion of contaminated food & water 3. Droplet infection
Dr.T.V.Rao MD Inhalation of droplets of infected urine 19
Transmission
Urine Tissue Feces
Contam
Survive
Infection
Animal Source
Environment
Human
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Culturing of Leptospira
Leptospira grows best under aerobic conditions at 280 to 300c best demonstrated in Semisolid agar media Optimal Media Fletchers Media Stuarts Media Optimal growth after 1 2 weeks
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Growth requirements
Leptospira derive energy from oxidation of long chain fatty acids, and cannot use or carbohydrates or amino acids as major energy source.
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Antigenic structure
All isolates of L.inttterogans from different parts of the world are serologically related and exhibit cross reactions in serologic tests. Overlapping of Antigens do occur in different species. Outer envelop contains large amount of Lipopolysaccharides ( LPS ) Antigenic structure varies from one strain to other This variation forms the basis of serologic classification
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Genome of Leptospira
L. interrogans serogroups Icterhaemorrhagiae consists of a 4.33 mega base large chromosome and a 359 kilo base small chromosome, totalling 4,768 predicted genes. A series of genes have been discovered that could potentially be related to adhesion. This genome differs from the two other pathogenic spirochete (Treponema palladium and Borrelia burgdorferi), though some similar genes are visible (CHGC, 2004).
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PATHOGENESIS
leptospira skin,mucosa Initial stage leptospiremia toxic symptoms
(1~3days) three symptoms: fever,myalgia,fatigue; three signs: conjunctival suffussion; muscle tenderness; enlargement of lymphonodes;
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Pathogenesis
Leptospira are present in the water bodies
Enter through breaks in the skin ( cuts and abrasions ) and mucous membranes
Enters through Mouth Nose Conjunctive Rarely enters though ingestion. Incubation period 1 2 weeks When multiples blood stream produces fever. May establish organ involvement in Kidney and Liver, May produce hemorrhage and necrosis in the tissues and initiates dysfunction of these organs
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Clinical Illnesses
Types
Hepato-renal syndrome
Hemorrhagic syndrome with ARF Atypical pneumonia syndrome Aseptic meningo-encephalitis Myocarditis, Chronic uveitis
Clinical Presentation
90% of Cases
Anicteric
Common, mild < 2% Mortality
Icteric
Rare, Severe 15% Mortality
10% of Cases
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Leptospira
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Weils Syndrome
Weil's syndrome is a severe form of leptospirosis that causes a continuous fever, stupor, and a reduction in the blood's ability to clot, which leads to bleeding within tissues. Blood tests reveal anaemia. By the third to sixth day, signs of kidney damage and liver injury appear. Kidney abnormalities may cause blood in the urine and painful urination. Liver injury tends to be mild and usually heals completely.
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Hepatitis - Leptospirosis
Hepatitis is the frequent complication Elevation of serum creatine phospholipase enzyme raise differentiates from Viral hepatitis where the enzyme is not raised
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Nephritis - Leptospirosis
Kidney involvement in animals produce chronic disease of the kidney and the infected animal starts shedding large number of Leptospira and main source of environmental contamination of bacteria and results I human infections Human urine also contain Spirochetes in the second and third week of infection
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Complications
Azotemia Oliguria Hemorrhage Purpura Hemolysis Gastrointestinal bleeding Hypoprothrombinemia and Thrombocytopenia
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Fever
Differential Diagnosis
Jaundice
Malaria, Viral hepatitis, Sepsis
Renal Failure
Malaria, Hanta virus, Sepsis
Meningitis
Bacterial / Viral causes
Hemorrhagic Fever
Dengue, Hanta virus, Typhus
Laboratory Tests
TC / DC / ESR / Hb / Platelet count Serum Bilirubin / SGOT/ SGPT Blood Urea, Creatinine & Electrolytes Chest X-Ray; ECG Tests for diagnosis of Leptospirosis
Culture for Leptospira: Positive MAT; Sero conversion or 4 fold rise/ high titer ELISA / MSAT : positive MAT: Microscopic agglutination test (M)SAT: Microscopic slide agglutination Test
Approach to Diagnosis
Clinical Features
Immune 7d
phase >
Blood
Culture
PCR
ELISA
MSAT
Repeat
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MAT
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Laboratory Diagnosis
Specimens
1 Blood
to be collected in a heparin tube 2 CSF, Tissues Microscopic examination 3 Urine to be collected with great care to avoid contamination 4 Serum for agglutination tests
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Culturing Leptospira
Blood and Urine be cultured in Fletchers semisolid agar or other media chemically defined protein-free media for the growth of leptospires have been proposed.
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1 week
1 month
2 months
1 year
5 years
ELISA or SAT
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4 1 15 15 15 25
Definite
Rain fall Contaminate H20 Animal contact ELISA IgM + ve SAT positive MAT high titer
Jaundice
Alb, creatinine
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Serology
Agglutinating antibodies raise to very high titers 1 : 10,000 or higher occurs 5 10 weeks after onset of infection
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Serology - ELISA
Several Immunoassays are available as commercial kits Detection of IgM and razing titers of IgG will guide in association with clinical history will help in Diagnosis
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Treatment
Antibiotic of choice is Benzyl Penicillin given by injection in doses of 5 mega units in a day, for 5 days. If the patients are genuinely hypertensive to Penicillin opted with Erythromycin 250mgs four times a day for a period of 5 days.
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Preferred treatment
Penicillin 6 million units daily I.V (10-14 days) Amoxicillin, Erythromycin, & Doxycycline Patients with MOF(Multi organ failure) to be observed and treated in intensive care unit
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Epidemiology
Rainfall; Contaminated environment Poor Sanitation; Inadequate drainage facilities Presence of rodents, cattle & stray dogs Walking/ working bare foot poses high risk Difficult to pinpoint the source of infection
Epidemiology
Leptospirosis causes several animal infections Most wide spread zoonotic infection in Nature Human infections are accidental associated with contamination of water, other materials contaminated with excreta and animal flesh. Animal carriers often excrete upto 100million leptospirosis per ml of urine
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Epidemiology - Occupation
Certain occupational groups such as agriculture workers in rice and cane fields, miners and sever cleaners are potential victims
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Control of Leptospirosis
Rodent control is most important. Humans should avoid contact with water contaminated with animal contact.
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Chemoprophylaxis
Doxycycline 200 mg orally once a week is simple effective measure. When heavy exposure is anticipated
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Vaccination in humans
Vaccination for humans is justified where they cannot be separated from animal sources or where the animals cannot be immunized successfully Necessity of human vaccinated will arise where people live and work in proximity to rodents in wet, tropical conditions, in wet rice planting and harvesting, in military operations, or working in sewers. Yet no universally accepted vaccine is available for humans
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Vaccination of Animals
Vaccinating animals have a dual purpose 1 Protecting animals 2 Protecting humans who may contract leptospirosis from them It is probably true as that immunization of animals will prevent leptospirosis in people in contact with them. It proved true in 1980 when extensive vaccination of dairy cows in New Zealand lead to marked decreased incidence in Humans. Animals immunized experimentally with polysaccharide derived from Leptospira LPS linked to diphtheria Toxoid were protected against challenges Several other vaccines in use to suit local needs.
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Prevention
Prevention is difficult due to wild animal infection Good sanitation, Immunization of live stock
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doctortvrao@gmail.com
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