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biodiversitatii
Importanta geneticii recunoscuta din anii 70
- Sir Otto Frankel colab cu Soule parintele biologiei
conservarii
Diversitatea genetica
Este indispensabila populatiilor pentru a se putea
adapta.
Populatiile mari ale speciilor panmictice diversitate
genetica mare
Diversitate redusa la sp. periclitate
Diversitatea alelica
Este folosita pentru a descrie diversitatea
genetica.
De ex.
Doua alele la un locus determina
diferente in proteinele albuminei din ou
la eider si trei alele la o sp. de cinteza
(ex. 2.2).
Daca se analizeaza mai multi loci,
diversitatea alelica (A) este numarul
mediu al alelelor la nivelul acestor loci
(Tab 2.1).
Ex leii africani au in total 33 de alele
In 26 loci alozimici deci A = 33/26 =
1.27.
Echilibrul HardyWeinberg
In populatiile panmictice mari, frecventele genice si genotipice la
un locus autozomal sunt in echilibru asu ating echilibrul dupa o
generatie de imperechere panmictica (in absenta factorilor
modificatori).
Variatii cantitative
Cele mai importante caractere
in conservare sunt cele
cantitative
Alele detrimentale
Diversitatea genetica
Proteine
Primele masuratori de diversitate
genetica la nivel molecular au fost
realizate in 1966 prin studiul
variatiilor alelice la nivelul unor loci
responsabili de sinteza unor proteine
solubile
Tehnica o varianta a electroforezei
care separa moleculele dupa
incarcatura lor electrica si masa
moleculara
ADN
Recoltarea probelor de ADN pentru masurarea diversitatii
genetice
Orice material biologic care contine ADN poate fi utilizat pentru masurarea
diversitatii genetice cu ajutorul tehnicilor moleculare
probe proaspete: par, piele, penaj, materii fecale, urina, coaja de oua,
solzi, tesuturi, saliva, lichid seminal.
material conservat tegument si tesuturi ale unor piese muzeale
Conditie proba trebuie sa contina si ADN nedegradat care sa nu fie
contaminat cu ADN al altor indivizi sau al unor specii inrudite.
Permite amplificarea in
laborator a unor secvente de
ADN specifice din probe mici
Diversitate mare
imperechere panmictica
The highest levels of genetic diversity in DNA are typically found in DNA
sequences with little functional significance.
These variants either do not code for functional products, or substitutions do not
change the function of the molecule, i.e. they are not expressed phenotypically.
Therefore, selection does not act against such changes.
Conversely, the lowest genetic diversity is found for functionally important
regions of molecules, such as the active sites of enzymes. Much of the DNA
in an organism does not code for functional products.
Mirounga angustirostris
Lasiorhinus krefftii
Potorous longipes
Onychogalea fraenata
Swietenia mahagoni
Canis simensis
Evolutionary genetics
of natural populations
global warming
translocations of species,
extinction of food species,
inadvertent or deliberate introduction of novel chemicals
to the environment
In animals:
In plants:
pollen production,
ability of pollen to reach the
stigma of flowers, germinate,
grow down the style and
fertilize,
number of ova,
viability of the fertilized zygotes,
their ability to disperse,
germinate and grow to sexual
maturity,
the fertility of the resulting plant.
Recessive lethal
chondrodystrophic
dwarfism
(dwdw)
Selection not only applies to lethals. Any allele that changes the
relative fitness of its carriers will be subject to selection. If the
effect on fitness is small then the change in allele frequency will be
correspondingly smaller.
Mutationselection balance
Selective value of mutations
As the majority of the genome is not expressed in the
phenotype(i.e. non-coding DNA), mutations in these
regions will not affect fitness and be selectively
neutral. These mutations are, however, invaluable
in conservation genetics as they provide genetic
markers (alleles that differ in frequency among
individuals or populations) such as microsatellites for
identifying individuals, populations and
species.
Mutations that alter the amino acid sequences of
proteins, but do not affect the physiological
functioning of the protein, are also selectively neutral
and provide markers (e.g. allozymes).
Deleterious mutations
occur at thousands of loci
in the genome, so their
cumulative rate is
relatively high
natural selection
favoured the wild-type
allele (+) over the mutant
black (b).
genetic drift has
overpowered this selection
and resulted in fixation
of b in one N = 10
population.
Single generation
bottlenecks have to be
severe before they
have a substantial impact
on heterozygosity.
A bottleneck of size 2 still
retains 75% of initial
heterozygosity.
Inbreeding
Inbreeding is unavoidable in
small populations and leads to
reductions in reproduction and
survival
Inbreeding is of profound importance in conservation biology as
it leads to reductions in heterozygosity, to reduced reproduction
and survival (reproductive fitness), and to increased risk of
extinction.
Ex. in a study of 44 captive mammal populations, inbred
individuals experienced higher juvenile mortality than outbred
individuals in 41 cases.
On average, brother--sister mating resulted in a 33% reduction
in juvenile survival.
Inbreeding
coefficient - F
each noninbred ancestor is labelled as
having unique alleles (A1A2, A3A4)
the probability that an individual inherits
two alleles identical by descent (A1A1,
A2A2, etc) is computed from the paths of
inheritance, assuming normal Mendelian
segregation.
Inbreeding accumulates
over time and is more rapid
in smaller than in larger
populations
Unequal sex-ratio
High variation
in family sizes in wildlife is
partly due to individuals that
contribute no offspring to the
next generation.
Similar but less extreme
effects arise from very large
and very small families.
Population fragmentation
The genetic consequences of population fragmentation depend
critically upon the level of gene flow.
With restricted gene flow, fragmentation is usually highly
deleterious in the long term
The gene flow depend on the:
number of population fragments
Measuring population
fragmentation: F statistics
Inbreeding resulting from population fragmentation can be used
to measure the degree of differentiation that has occurred among
fragments.
Differentiation among fragments or sub-populations is
directly related to the inbreeding coefficients within and among
populations.
The inbreeding in the total population (FIT) can be partitioned
into that due to:
inbreeding of individuals relative to their sub-population or fragment,
FIS
inbreeding due to differentiation among sub-populations, relative
to the total population, FST.