SEPARAREA I CARACTERIZAREA

ANUMITOR ANTIOXIDANI DIN FRUCTELE DE CTIN

Autor: Paula GROZA
Coordonator tiinific: Andrea BUNEA
Universitatea de tiine Agricole i Medicin Veterinar, Str. Mntur, Nr. 3-5,
400372 Cluj-Napoca, Romnia; paula.groza@ymail.com

REZUMAT
Ctina, Hippophae rhamnoides L., este extrem de cunoscut datorit faptului c fructele
sale sunt foarte bogate n substane chimice: lipide, acizi grai nesaturai i saturai, vitamina C,
vitamina E, steroli, flavonoide. Coninutul bogat n vitaminele D, E i n carotenoide confer
fructelor de ctin calitatea de surse de antioxidani. Fructele de ctin sunt utilizate n medicin
pentru tratamentul ulcerului gastic, pentru proprietile hepato-protectoare i antitumorale, pentru
stimularea imunitii; n industia farmaceutic pentru obinerea diferitelor preparate; n industria
alimentar ca pigmeni alimentari.Culoarea galben-portocalie a fructelor de ctin se datoreaz,
n principal, carotenoidelor existente n pulpa acestora. Coninutul de carotenoide n fructele de
ctin variaz de la 0,2-17 mg/100 g n funcie de varietatea studiat, de factorii climatici i de
condiiile de cultivare. Pentru a determina i doza compuii chimici se folosesc urmtoarele
metode: cromatografia de gaze (GC), cromatografia lichid de nalt performan (HPLC), GCspectometria de mas (GC-MS) i HPLC-spectometria de mas (HPLC-MS). Scopul lucrrii este
de a determina coninutul n vitamina C, vitamina E i carotenoide din diferite varieti de fructe
de ctin. Metoda folosit pentru dozarea vitaminei C este o metod volumetric, carotenoidele
i vitamina E se vor identifica folosind cromatografia lichid de nal performan.

CUVINTE CHEIE
Ctin, vitamina C, carotenoide, vitamine E
BIBLIOGRAFIE
Cakir Ahmet, 2004, Essential oil and fatty acid composition of the fruits of Hippophae
rhamnoides L. and Myrtus communis L. from Turkey. Bioch.System and Ecology 32:809816.
Kallio, H., et al., 2002, Triacylglycerols, glycerophospholipids, tocopherols and
tocotrienols in sea buckthorn Hippophae rhamnoides L. ssp. sinensis and ssp. mongolica berries
and seeds. J. Agric. and Food Chem., 50:3004-309.

THE SEPARATION AND IDENTIFICATION

OF CERTAIN ANTIOXIDANTS IN SEABUCKTHORN BERRIES
Author: Paula Groza
Scientific Coordinator: Andrea Bunea
University of Agricultural Sciences and Veterinary Medicine, 3-5 Manastur St.,
400372 Cluj-Napoca, Romania; paula.groza@ymail.com

ABSTRACT
Sea Buckthorn, Hippophae rhamnoides L., is well-known all over the world because its
fruits are rich in chemical substances: fats, unsaturated and saturated fatty acids, vitamin C,
vitamin E, sterols, flavonoids. The rich content in vitamin D and E and in carotenoids gives to
the Sea Buckthorn fruits the quality of antioxidants sources. As a result of their chemical content,
Sea Buckthorn fruits are used in medicine for the treatment of gastric ulcer, for their hepatoprotective and antitumor properties, and as stimulators of the immune system; in pharmaceutical
industry for production of certain medicines; in food industry as food pigments. The yelloworange color of the fruits its due to the carotenoids pigments, which are contained by the pulp of
the fruit. The carotenoids content in seabuckthorn fruits are between 0,2 and 17mg/100g
depending on the variety studied, climatic factors and growing conditions. In order to determine
the chemical composition there were used the following methods: gas chromatography (GC),
high performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GCMS) and HPLC-MS. The aim of this paper is the determination of the amount of vitamin C,
vitamin E and carotenoids in several varieties of Sea Buckthorn fruits. The method used for
determination of vitamin C is a volumetric method, and the carotenoids and vitamin E will be
identified using HPLC technique.
KEY WORDS
Seabuckthorn berries, carotenoids, vitamin C and vitamin E
REFERENCES
Cakir Ahmet, 2004, Essential oil and fatty acid composition of the fruits of Hippophae
rhamnoides L. and Myrtus communis L. from Turkey. Bioch.System and Ecology 32:809816.
Kallio, H., et al., 2002, Triacylglycerols, glycerophospholipids, tocopherols and
tocotrienols in sea buckthorn Hippophae rhamnoides L. ssp. sinensis and ssp. mongolica berries
and seeds. J. Agric. and Food Chem., 50:3004-309.