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Chapman Agar 13d26285892
Chapman Agar 13d26285892
g/l
5.00
5.00
1.00
10.00
75.00
0.025
15.00
METODA
Se suspend 111 grame de pulbere ntrun litru de ap purificat. Se fierbe sub
omogenizare constant pn la dizolvarea
complet. Se toarn n tuburi sau flacoane. Se
autoclaveaz 15 minute la 121C.
PROCEDURA
Se lichefiaz mediul n ap fierbinte, se
rcete apoi la 45-50C (n conformitate cu
specificaiile standard) ntr-o a doua baie de ap.
Se toarn mediul n plci Petri, lasnd plcile pe
o suprafa orizontal.
Bacteriologie medical:
Se nsmneaz suprafaa plcii cu
proba. Se ntorc plcile invers nainte de incubare
la 36 2C. Culturile se examineaz n general
dup 24 pn la 48 de ore de la incubare.
Metoda armonizat a farmacopeei:
Se nsmneaz o plac de Chapman cu
proba mbogit. Se ntorc plcile invers nainte
de incubare la 32.5 2.5C pentru 18 pn la 72
de ore.
Analize de ap : (n conformitate cu
standardul francez)
Se filtreaz o prob cu un volum
corespunztor printr-o membran cu porii de
0.45m. Se transfer membrana pe o plac cu
Chapman. Nu trebuie s existe aer ntre
membran i agar. Se ntoarce placa invers
nainte de incubarea la 36 2C timp de 44 4
ore.
REZULTATE
Stafilococii patogeni cresc n colonii
abundente, producndu-i propriul lor pigment.
Aceste colonii sunt nconjurate de un halou
galben, ca urmare a fermentrii manitolului.
Stafilococii nepatogeni cresc n principal
sub forma unor colonii mici i roii.
PROPRIETI
Temperatura de incubare a agarului este
aleas n conformitate cu procedura i
reglementrile n vigoare. Tulpinile de
SC Mikrobiologie Labor-Technik SRL P-ta. Heim Domokos, nr. 19, parter 310103 ARAD Romania
Telefon/Fax: +40 (0)257 27 98 11 E-mail: office@mlt.eu.com Web: www.mlt.eu.com
SC Mikrobiologie Labor-Technik SRL P-ta. Heim Domokos, nr. 19, parter 310103 ARAD Romania
Telefon/Fax: +40 (0)257 27 98 11 E-mail: office@mlt.eu.com Web: www.mlt.eu.com
PRINCIPLE
Chapman Agar (Mannitol Salt Agar)
is used for the selective isolation and
identification of Staphylococci in all types of
samples. This medium is compliant with the
formula described by Chapman, it contains a
high level of Sodium Chloride (75 g/l) which
inhibits the growth of most interfering flora
(except a few Bacillus, Corynebacterium &
Streptococcus faecalis). Acid productions
resulting from the mannitol fermentation cause
a colour change of the pH indicator, phenol red,
from red to yellow. Pathogen Staphylococci,
coagulase positive, grow as larges colonies
surrounded by a yellow halo. Staphylococci
coagulase negative colonies are small and do
not cause a change of colour.
FORMULA
Components
Tryptone
Peptic digest of animal tissue
Meat extract
Mannitol
Sodium chloride
Phenol red
Agar
Final pH : 7.4 0.2 at 25C
g/l
5.00
5.00
1.00
10.00
75.00
0.025
15.00
METHOD
Suspend 111 grammes of powder in one
litre of purified water. Bring to the boil under
constant homogenisation until completely
dissolved. Dispatch in tubes or flasks.
Autoclave 15 minutes at 121C.
PROCEDURE
Liquefy the medium in boiling water
then cool it to 45-50C (according to standard
SC Mikrobiologie Labor-Technik SRL P-ta. Heim Domokos, nr. 19, parter 310103 ARAD Romania
Telefon/Fax: +40 (0)257 27 98 11 E-mail: office@mlt.eu.com Web: www.mlt.eu.com
PRECAUTIONS
It is important not to dry the medium in
an incubator since this might increase the
sodium chloride concentration making the
medium to selective.
BIBLIOGRAPHY
1. Chapman G.H. 1945. The significance
of sodium chloride in studies of
staphylococci. J. Bact. 50:201-203.
2. Chapman G.H. 1948. An improved
Stone medium for the isolation and
testing of food poisonning
staphylococci. Food Research, 13:100105.
3. The United States Pharmacopeia.
SC Mikrobiologie Labor-Technik SRL P-ta. Heim Domokos, nr. 19, parter 310103 ARAD Romania
Telefon/Fax: +40 (0)257 27 98 11 E-mail: office@mlt.eu.com Web: www.mlt.eu.com