Rspopescu Diana, seria B, gr.

10
Tomit Georgiana, seria A, gr. 2
Universitatea de Medicina si Farmacie
Victor Babes Timisoara


Efectul nanoparticulelor de aur asociate cu
unde electromagnetice asupra celulelor
maligne
Scopul studiului
Gasirea unei metode pt distrugerea celulelor
maligne
Caracteristici :
- neinvaziva
- viabila pt distrugerea celulelor maligne
- specificitate mare
- toxicitate redusa


Unde electromagnetice si
nanoparticulele de aur

Celelele maligne pot fi distruse termic datorita
undelor electromagnetice amplificate din mediu.

De ce nanoparticule de aur?

Ipoteza de nul a studiului:


1. GNPs-citotoxice
2. Celulele se dezvolta letal
3. Caldura produce injurii
Metode
Laborator: GNPs 5nm-metode biochimice,
filtrare (filtru de 0.22u).
In vitro 4 serii celulare maligne: Hep3B, Panc-1.
Serii control doar RF/serii RF+particule GNPs.
Studiu case contol/ test t pereche pt.:

Diferite concentratii de GNPs-1, 10, 67 ul/l-Expunere la RF: 1, 2, 5 min, Analiza ME.

Rezultate-citotoxicitatea
Viabilitate celule normale (fct. de C% GNPs):
Hep3B:


Panc-1:


1uM/l => 1005%
10uM/l =>986%
67uM/l => 86 8%

1uM/l => 9810%
10uM/l =>9011%
67uM/l => 81 9%

Rezultate-celule maligne distruse

Cel. maligne distruse
-doar RF:

-Pt GNPs(67uM/l)+RF-1,2,5 min~ 98%

Pt. 67um/l GNP+RF / RF => p=0.001
1 min- <20%
2min- <27%
Valoarea P

GNPs + RF

Discutie
RFA conventional

Studii anterioare

GNPs-avantaje

De urmarit pe viitor


Discutie
RFA conventional

Studii anterioare

GNPs-avantaje

De urmarit pe viitor


Discutie
RFA conventional

Studii anterioare

GNPs-avantaje

De urmarit pe viitor


Discutie
RFA conventional

Studii anterioare

GNPs-avantaje

De urmarit pe viitor


Concluzii
GNPs nu prezinta efect citotoxic sau
antiproliferativ pentru celulele umane.
GNPs asociate cu expunerea RF determina
distrugerea termica celulelor maligne din
tumorile gastrointestinale.



Efectul GNPs+RF

Referinte
1. Bilchik AJ, Wood TF, Allegra D, TsioulRamming KP, Morton DL:
Cryosurgical ablation and radiofrequency ablation for unresectable
hepatic malignant neoplasms: a proposed algorithm.
2. Bleicher RJ, Allegra, Bilchik AJ: Radiofrequency ablation in 447
complex unresectable liver tumors: lessons learned.
3. Curley SA, Marra P, Beaty Early and late complications after
radiofrequency ablation of malignant liver tumors in 608 patients.
4. Haemmerich D, Laeseke PF: Thermal tumour ablation: devices,
clinical applications and future directions.
5. Gannon CJ, Curley SA: The role of focal liver ablation in the treatment
of unresectable primary and secondary malignant liver tumors.
6. Raut CP, Izzo F, Marra P, Ellis LM, Vauthey JN, Cremona F, Curley SA:
Significant long-term survival after radiofrequency ablation of
unresectable hepatocellular carcinoma in patients with cirrhosis.
Va multumim!