Bioinformatica- aplicatii in patologiile virale

La virusuri, genomul este reprezentat de molecula de

•ADN (Adenovirusurile)
ex: virusul herpetic
•ARN (RETROVIRUSURILE)
ex: virusul gripal, HIV, SARS,EBOLA,SARS

PROTEINE VIRALE -TINTE

BIOINFORMATICE
1.GLICOPROTEINELE (EX GP120,
GP41)
2.REVERSTRANSCRIPTAZA-RT
3.PROTEAZA VIRALA-PRO
4.INTEGRZA-INT
BAZE DE DATE UTILE IN STUDIUL VIRUSURILOR
https://www.ncbi.nlm.nih.gov/nuccore/1798174254
Genomul complet pr CoVID19 identificat accesand adresa de mai sus
https://www.ncbi.nlm.nih.gov/nuccore/NC_045512.2?report=fasta
Accesarea fisierului FASTA CoVID19-selectiv
 O alta baza utila in studiul proteinelor si genelor virale este UNIPROT
https://www.uniprot.org/
 Baza de date permite identificarea structurală, funcțională sau de
mutageneză a structurilor proteice virale. Sunt informatii complexe despre-
genele care sunt implicate in translatia proteinelor virale, lungimea (numarul
de amino acizi) proteinelor, mutatii care duc la activarea sau inhibarea
virusului, etc.

Site-ul este coordonat de- European Bioinformatics Institute (EBI),

Swiss Institute of Bioinformatics(SIB) și Protein Information Resource (PIR).

Ce informatii gasim in aceasta baza de date-

Nume de proteine și gene
Funcţia proteinelor
Informații structural si functionale pentru protein: activitatea catalitică, cofactori și
reziduuri catalitice
Interacțiuni proteină-proteine
Centre catalytic active
Mutatii punctiforme sau multiple in secventa proteinelor virale, etc
Informatii utile putem identifica, prin utilizarea bazei de date, atunci cand dorim sa studiem
omologii structurale intre structurile proteice virale. Putem analiza de exemplu, genele
virale SARS 2, proteinele specifice si gazdele specifice.
2. VIRUS-HOST DB- Alta baza de date ,foarte utila in studiul patologiilor virale
http://www.genome.jp/virushostdb
Aceasta baza de date ne ofera informatii structurale, taxonomice, moleculare si de
interactiune dintre virusuri și gazdele lor.
Baza de date cuprinde un numar mare de clase virale (vezi imaginea de mai
jos).Identificam virusuri din clasa adeno, retro, Herpesviridae, coronaviridae, etc.
Informațiile despre gazdele virale sunt colectate de la RefSeq, GenBank (în format
text gratuit), UniProt, ViralZone PubMed
3. Virus Pathogen Database and Analysis
Resource-alta baza de date ce contine informatii virale
https://www.viprbrc.org/brc/home.spg?decorator=vipr
The Virus Pathogen Database and Analysis Resource (ViPR) is an integrative and
comprehensive publicly available database and analysis resource to search, analyze,
visualize, save and share data for viral pathogens in the U.S.
National Institute of Allergy and Infectious Diseases (NIAID) Category A-C Priority
Pathogen lists for biodefense research, and other viral pathogens causing
emerging/reemerging infectious diseases. ViPR is one of the five
Bioinformatics Resource Centers (BRC) funded by NIAID, a component of the
National Institutes of Health (NIH), which is an agency of the
United States Department of Health and Human Services.

Data types in ViPR[edit]

•Genomes
•Genome annotations
•Genes & proteins
•Predicted protein domains and motifs
•Immune epitopes
•Sequence Features [4]
•Orthologous protein clusters
•3D protein structure
•Clinical metadata
•Host factor data
4.HIV DATABASES
https://www.hiv.lanl.gov/content/index
 De citit

A New Era of Virus Bioinformatics

•May 2018
•Virus Research 251
•DOI:
•10.1016/j.virusres.2018.05.009

https://www.drugtargetreview.com/news/57287/3d-visualisation-of-covid-19-surface-
released-for-researchers/

https://academic.oup.com/bioinformatics/advance-article/doi/10.1093/
bioinformatics/btaa145/5766118