Medicina generala - UMF Carol Davila

PROTOZOARE

Asistent universitar NEAGOE IONELA

AMIBE

AMIBE PATOGENE

Entamoeba hystolitica

Entamoeba hystolitica
Stadiul de CHIST

Examen coproparazitologic = Frotiu din fecale - coloratie Tricrom

Entamoeba hystolitica
Trophe (gr)=
hranire; zoon
(gr)= animal

Examen
coproparazitologic =
Frotiu din fecale
muco-piosangvinolente coloratie Giemsa

Stadiul de TROFOZOIT

Entamoeba hystolitica
Colonoscopie - Abcese primare in buton de camasa

Entamoeba hystolitica
Abces primar in buton de camasa

Examen histopatologic = Sectiune tesut intestinal - coloratie H&E

Entamoeba hystolitica
Abces secundar amoebian - hepatic

Entamoeba hystolitica
Abces secundar amoebian -perianal

AMIBE COMENSALE

Entamoeba coli
Stadiul de CHIST

Examen
coproparazitologic
= Preparat proaspat
L+l din fecale +
solutie Lugol

8 nuclei in
planuri
suprapuse

AMIBE LIBERE CONDITIONAT

PATOGENE

1. Naegleria fowlery
2. Acanthamoeba sp.

Naegleria fowlery

Naegleria fowlery
Stadiul de CHIST

Naegleria fowlery
Stadiul FLAGELAT

Flageli
(2)

Naegleria fowlery
Stadiul de TROFOZOIT

Recunoastere dupa nucleul cu aspect de tinta

Examen histopatologic =
Sectiune tesut cerebral
coloratie H&E

Acanthamoeba sp.

Acanthamoeba sp.
Stadiul de CHIST

Preparat
proaspat
L+l din
suspensie
tesut de
raclaj
corneean

Acanthamoeba sp.
Stadiul de TROFOZOIT

Nucleu

Preparat
proaspat
L+l din
LCR

Acantopodii

Acanthamoeba sp.
Keratita amoebiana

Acanthamoeba sp.

Abcese subcutanate granulomatoase (la

imunosupresati)

Acanthamoeba
sp.
Encefalita granulomatoasa amoebiana
cronica (la imunosupresati)

FLAGELATE

FLAGELATE CAVITARE
1. Giardia duodenalis
2. Trichomonas vaginalis

Giardia duodenalis

Giardia duodenalis
Stadiul de CHIST

Examen
coproparazitologic
= Preparat proaspat
L+l din fecale formate
+ Solutie Lugol

Giardia duodenalis
Stadiul de TROFOZOIT

Examen
coproparazitolo
gic =
Frotiu fecale
diareice coloratie Giemsa

Giardia duodenalis
Stadiul de TROFOZOIT

Ventuza/Disc adeziv
Instalare MALABSORTII

Trichomonas vaginalis

Trichomonas vaginalis
Stadiul de TROFOZOIT

Frotiu secretie - coloratie Giemsa

Trichomonas vaginalis
Coposcopie - Vaginita + Leucoree - FAZA INCIPIENTA

Trichomonas vaginalis
Colposcopie - Strawberry cervix + coinfectie cu Candida

Trichomonas vaginalis

Testul InPouch TV pentru

detectia directa si cultivarea
trofozoitilor

Trichomonas vaginalis

Test rapid imunocromatografic - detecti

antigene

HEMOFLAGELATE
1.

Leishmania sp.

2. Trypanosoma brucei
3. Trypanosoma cruzii

Leishmania sp.

Leishmania sp.
Phlebotomus
sp

(Sandfly)
Lutzomia sp.

Leishmania sp.
Stadiul PROMASTOGOT

Mastigot =
kinetida (gr) =
flagel+
corpuscul
bazal/kinetosom

Flagel liber

kinetoplast

Frotiu GLANDE
SALIVARE
MUSCULITA DE
NISIP - coloratie
GIEMSA

nucleu

Leishmania sp.
Stadiul AMASTOGOT

Amastigot uninucleat

kinetoplast

Nucleu
macrofag

Frotiu aspirat
medular coloratie
Giemsa

Macrofag

Leishmania sp.
Buton de Orient
- Forma uscata - L. tropica

Leishmania sp.
Buton Orient - Forma umeda - L. major

Leishmania sp.
Forma ESPUNDIA - L. brasiliensis

Leishmania sp.
Ulcerul chiclero- L. mexicana

Leishmania sp.
L. donovani
L. Infantum
L. chagasi

Boala Kalaazar, febra

Dum-Dum,
boala
neagra FAZA
CRONICA

Leishmanioza cutanata
post kala-azar - FAZA
CRONICA

Leishmania sp.
Leismania donovani

Trypanosoma brucei

Trypanosoma
brucei
Glossina sp. ( musca tze-tze )

Trypanosoma brucei
Stadiul EPIMASTOGOT

Membrana ondulanta
scurta

Flagel
liber
anterior

Frotiu din
cultura
dezvoltata
pe mediul
NNN coloratie

Nucleu

GIEMSA

Trypanosoma brucei
Stadiul TRIPOMASTIGOT

Membrana
ondulanta lunga

Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA

Flagel liber
anterior

Corpuscul bazal /Kinetosom

Kinetoplast

Nucleu

Trypanosoma brucei
gambiense
Sleeping sickness (Boala somnului)
FAZA INCIPIENTA

Semnul Winterbottom

Trypanosoma brucei
Sleeping sickness (Boala
somnului) - FAZA CRONICA

Meningoencefalita cronica

Trypanosoma cruzii

Trypanosoma cruzii

Triatoma sp. (Kissing bug)

Reduviid bug

Trypanosoma cruzii
Stadiul TRIPOMASTIGOT

Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA

Trypanosoma cruzii
Stadiul AMASTOGOT

Amastogoti
uninucleati

Celula
nervoasa

Aprenta tesut cardiac coloratie Giemsa

Trypanosoma cruzii
Boala Chagasi (FAZA INCIPIENTA)

Semnul Romana

Trypanosoma cruzii

Boala Chagasi (FAZA CRONICA)

Cardiomiopatie cu
megalie cardiaca

Anevrism
cardiac

Trypanosoma cruzii
Boala Chagasi (FAZA CRONICA)

Megacolon

SPOROZOARE

Sporozoare tisulare

1. Toxoplasma gondii

Toxoplasma gondii

Toxoplasma gondii
Stadiul de OOCHIST
P.C.
Sporozoiti
uninucleati

Sporochisti

Examen coproparazitologic - fecale PISICA

Toxoplasma gondii
Stadiul de TACHIZOIT (trofozoitul de Toxoplasma)

Frotiu
ascita
soarece
infectat coloratie
Giemsa

TACHIZOITI uninucleati

Macrofag
tachys (gr)=rapid; zoon (gr)= animal

Toxoplasma gondii
Stadiul de CHIST TISULAR cu bradizoiti
Celula
nervoasa

BRADIZOITI

bradys (gr)=lent; zoon (gr)= animal

Amprenta
tesut
cerebral
coloratie
Giemsa

Toxoplasma gondii
Hidrocefalie

Toxoplasma gondii

Corioretinita
(la imunosupresati si la
nou-nascuti cu
toxoplasmoza
congenitala)

Toxoplasma gondii
Neurotoxoplasmoza (la imunosupresati)

CT

Sporozoare tisulare
2. Plasmodium vivax
Plasmodium ovale
Plasmodium malariae
Plasmodium falciparum

Plasmodium sp.

Plasmodium sp.
Tantarul Anopheles sp.

Plasmodium sp.
OOCHISTI fixati pe fata
externa a stomacului unui
tantar infectat.

Plasmodium sp.
SPOROZOITI

Frotiu GLANDE SALIVARE TANTAR - coloratie GIEMSA

Plasmodium sp.
Amprenta
tesut
hepatic
coloratie
Giemsa

MEROZOITI

INTRAHEPATOCITARI

Plasmodium sp.
Plasmodium vivax
INEL= TROFOZOIT TANAR

inel

Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA

Nucleu
Vacuola
centrala
Citoplasma
putina,
periferica

Plasmodium sp.
Plasmodium vivax
AMOEBA = TROFOZOIT MATUR

Frotiu SANGE
PERIFERIC
coloratie
GIEMSA

Examen
hematologic =
Citoplasma
bogata, cu
multe
prelungiri

Nucleu
amoeba

Plasmodium sp.
Plasmodium vivax
ROZETA = SCHIZONT MATUR
16-24 merozoiti
Granulatii
Schuffner
hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA

Examen
Nuclei
merozoiti

Corp muriform

Plasmodium sp.
Plasmodium vivax
GAMETOCIT MASCULIN

Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA

Granulatii
Schuffner

Nucleu
central
nedefinit

Plasmodium sp.
Plasmodium vivax
GAMET
OCIT FEMININ
Schuffner
Granulatii
Frotiu
SANGE
PERIFERIC
coloratie
GIEMSA
Nucleu gametocit periferic, triunghiular

Plasmodium sp.
Plasmodium ovale
INEL= TROFOZOIT TANAR
Nucleu inel

Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA

Plasmodium sp.
Plasmodium ovale
AMOEBA = TROFOZOIT MATUR

Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA

Nucleu
amoeba

Plasmodium sp.
Plasmodium ovale
ROZETA = SCHIZONT MATUR
8-12 merozoiti

Frotiu
SANGE
PERIFERIC
coloratie
GIEMSA

Nuclei
merozoiti

Plasmodium sp.
Plasmodium ovale
GAMETOCIT MASCULIN

PERIFERIC
coloratie
GIEMSA

Examen
hematologic =
Frotiu SANGE
Granulatii
Schuffner /
James

Plasmodium sp.
Plasmodium ovale
GAMETOCIT FEMININ
Granulatii
Schuffner /
James

coloratie
GIEMSA

Examen
hematologic =
Frotiu SANGE
PERIFERIC
Nucleu gametocit periferic, triunghiular

Plasmodium sp.
Plasmodium malarie
INEL= TROFOZOIT TANAR

PERIFERIC
coloratie

Examen
hematologic =
Frotiu SANGE
Nucleu
inel
GIEMSA

Plasmodium sp.
Plasmodium malarie
AMOEBA = TROFOZOIT MATUR
Amoeba in banda
Granulatii
Ziemann

Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA

Nucleu
amoeba

Plasmodium sp.
Plasmodium malarie
ROZETA = SCHIZONT MATUR
8-12 merozoiti
Rozeta in margareta

Granulatii
Ziemann

merozoiti

Nuclei
Nuclei
granulocit

Examen hematologic = Frotiu SANGE Examen hematologic = Picatura groasa

PERIFERIC coloratie GIEMSA
SANGE PERIFERIC coloratie GIEMSA

Plasmodium sp.
Plasmodium malarie
GAMETOC
IT MASCULIN
Ziemann
Granulatii
Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA

Plasmodium sp.

Granulatii
Ziemann

Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA

Plasmodium malarie
GAMETOCIT FEMININ

Nucleu gametocit periferic, triunghiular

Plasmodium sp.
Plasmodium falciparum
INEL= TROFOZOIT TANAR

Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA

Hematie
multiparazitata
cu inele

Inele uni si bi nucleate

FAZA INCIPIENTA

Plasmodium sp.
Plasmodium falciparum
AMOEBA = TROFOZOIT MATUR

Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie GIEMSA

FAZA COMATOASA

Plasmodium sp.
Plasmodium falciparum
ROZETA = SCHIZONT MATUR
8-32 merozoiti
Merozoiti
uninucleati

Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA

granulatii
Maurer
FAZA COMATOASA

Plasmodium sp.
Plasmodium falciparum
GAMETOCIT MASCULIN in cosulet

hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA

Examen
Nucleu
gametocit

FAZA INCIPIENTA

granulatii
Maurer

Plasmodium sp.
Plasmodium falciparum
GAMETOCIT FEMININ in cosulet

Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA
FAZA INCIPIENTA

Plasmodium sp.
Plasmodium falciparum

GAMETOCIT
MASCULIN

INEL

Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA

GAMETOCITI FEMININI

FAZA INCIPIENTA

Plasmodium falciparum
Neuropaludism - Malarie cerebrala

Pete hemoragice
(petesii in rozeta)
= semn specific in
malaria cerebrala

Test rapid bazat pe

detectie Ag HRP2 de P.f

Teste imunocromatografice
pentru detectia antigenelor
malarice

Test rapid bazat pe detectie dubla Ag

pLDH de P.f si P,v

Sporozoare digestive
3. Cryptosporidium parvum
Cryptosporidium hominis

Cryptosporidium sp.

Cryptosporidium sp.
Stadiul de OOCHIST

Sporozoiti
uninucleati
(4)

Perete celular

Examen coproparazitologic = Frotiu fecale coloratie ZIEHL-NIELSEN modificata

Sporozoar digestiv

4. Isospora belli

Isospora belli

immature

Isospora belli
Stadiul de OOCHIST

Examen coproparazitologic
Oochist
matur

Oochist
imatur

(8) Sporozoiti
uninucleati

(2)
Sporochisti

Preparat
proaspat L+ l
din fecale cu SF

Frotiu fecale coloratie

ZIEHL-NIELSEN
modificata

Sporozoar - Transfug
5. Pneumocystis carinii
Pneumocystis jiroveci

Pneumocystis carinii
Pneumocystis jiroveci
Cale
aerogena

Pneumocystis carinii

TROFOZOITI (nuclei trofozoiti in nor cu puncte)

Frotiu LBA coloratie GIEMSA.

Pneumocystis carinii

TROFOZOITI
CHISTI
cu (8)
corpusculii
colorati

Frotiu LBA coloratie GIEMSA.

Pneumocystis carinii
CHISTI cu perete
celular colorat

Frotiu LBA coloratie Albastru de Toluidina.

Pneumocystis carinii

RX- plamani in geam mat

Pneumocitoza P.c interstitiala plasmocitara