PROTOZOARE

Asistent universitar NEAGOE IONELA

Medicina generala UMF Carol Davila
AMIBE
AMIBE PATOGENE
Entamoeba hystolitica
Entamoeba hystolitica
Stadiul de CHIST
Examen coproparazitologic = Frotiu din fecale coloratie Tricrom
Entamoeba hystolitica
Stadiul de TROFOZOIT
Trophe (gr)=
hranire; zoon
(gr)= animal
Examen
coproparazitologic =
Frotiu din fecale
muco-pio-
sangvinolente
coloratie Giemsa
Entamoeba hystolitica
Colonoscopie Abcese primare in buton de camasa
Entamoeba hystolitica
Abces primar in buton de camasa
Examen histopatologic = Sectiune tesut intestinal coloratie H&E
Entamoeba hystolitica
Abces secundar amoebian - hepatic
Entamoeba hystolitica
Abces secundar amoebian -perianal
AMIBE COMENSALE
Entamoeba coli
Stadiul de CHIST
Examen
coproparazitologic
= Preparat proaspat
L+l din fecale +
solutie Lugol
8 nuclei in
planuri
suprapuse
AMIBE LIBERE CONDITIONAT
PATOGENE
1. Naegleria fowlery
2. Acanthamoeba sp.
Naegleria fowlery
Naegleria fowlery
Stadiul de CHIST
Naegleria fowlery
Stadiul FLAGELAT
Flagel
(2)
Naegleria fowlery
Examen histopatologic =
Sectiune tesut cerebral
coloratie H&E
Stadiul de TROFOZOIT
Recunoastere dupa nucleul cu aspect de tinta
Acanthamoeba sp.
Acanthamoeba sp.
Stadiul de CHIST
Preparat
proaspat
L+l din
suspensie
tesut de
raclaj
corneean
Acanthamoeba sp.
Stadiul de TROFOZOIT
Nucleu
Acantopodii
Preparat
proaspat
L+l din
LCR
Acanthamoeba sp.
Keratita amoebiana
Acanthamoeba sp.
Abcese subcutanate granulomatoase (la imunosupresati)

Encefalita granulomatoasa amoebiana cronica (la imunosupresati)
Acanthamoeba sp.
FLAGELATE
FLAGELATE CAVITARE
1. Giardia duodenalis
2. Trichomonas vaginalis
Giardia duodenalis
Giardia duodenalis
Examen
coproparazitologic
= Preparat proaspat
L+l din fecale formate
+ SF
Stadiul de CHIST
Giardia duodenalis
Stadiul de TROFOZOIT
Examen
coproparazitolo
gic =
Frotiu fecale
diareice
coloratie Giemsa
Giardia duodenalis
Instalare MALABSORTII
Ventuza/Disc adeziv
Stadiul de TROFOZOIT
Trichomonas vaginalis
Trichomonas vaginalis
Stadiul de TROFOZOIT
Frotiu secretie coloratie Giemsa
Trichomonas vaginalis
Coposcopie Vaginita + Leucoree FAZA INCIPIENTA
Trichomonas vaginalis
Colposcopie Strawberry cervix + coinfectie cu Candida
Trichomonas vaginalis
Testul
InPouch
TV pentru
detectia
directa si
cultivarea
trofozoitilor
Test rapid imunocromatografic detectie antigene
Trichomonas vaginalis
HEMOFLAGELATE
1. Leishmania sp.

2. Trypanosoma brucei
3. Trypanosoma cruzii
Leishmania sp.
Leishmania sp.
Lutzomia sp. Phlebotomus sp
(Sandfly)
kinetoplast
Flagel liber
nucleu
Leishmania sp.
Stadiul PROMASTOGOT
Frotiu GLANDE
SALIVARE
MUSCULITA DE
NISIP - coloratie
GIEMSA
Mastigot =
kinetida (gr) =
flagel+
corpuscul
bazal/kinetosom
Leishmania sp.
Stadiul AMASTOGOT
Amastigot uninucleat
kinetoplast
Frotiu aspirat
medular
coloratie
Giemsa
Macrofag
Leishmania sp.
Buton de Orient
- Forma uscata - L. tropica

Leishmania sp.
Buton Orient - Forma umeda L. major
Leishmania sp.
Forma ESPUNDIA L. brasiliensis
Leishmania sp.
Ulcerul chiclero- L. mexicana

Leishmania sp.
Boala Kala-azar, febra Dum-Dum, boala neagra FAZA CRONICA
L. donovani
L. Infantum
L. chagasi
Leishmania sp.
Leishmanioza cutanata post kala-azar FAZA CRONICA
Leismania donovani
Trypanosoma brucei
Trypanosoma brucei
Glossina sp. ( musca tze-tze )
Trypanosoma brucei
Stadiul EPIMASTOGOT
Frotiu din
cultura
dezvoltata
pe mediul
NNN -
coloratie
GIEMSA
Nucleu
Membrana ondulanta
scurta
Flagel
liber
anterior
Trypanosoma brucei
Stadiul TRIPOMASTIGOT
Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA
Membrana
ondulanta lunga
Corpuscul bazal /Kinetosom
Kinetoplast
Nucleu
Flagel liber
anterior
Trypanosoma brucei gambiense
Sleeping sickness (Boala somnului) FAZA INCIPIENTA
Semnul Winterbottom
Trypanosoma brucei
Sleeping sickness (Boala somnului) FAZA CRONICA
Meningoencefalita cronica
Trypanosoma cruzii
Trypanosoma cruzii
Triatoma sp. (Kissing bug)
Reduviid bug
Trypanosoma cruzii
Stadiul TRIPOMASTIGOT
Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA
Trypanosoma cruzii
Stadiul AMASTOGOT
Amastogoti
uninucleati
Celula
nervoasa
Aprenta tesut cardiac
coloratie Giemsa
Trypanosoma cruzii
Semnul Romana
Boala Chagasi (FAZA INCIPIENTA)
Trypanosoma cruzii
Boala Chagasi (FAZA CRONICA)
Anevrism
cardiac
Cardiomiopatie cu
megalie cardiaca
Trypanosoma cruzii
Boala Chagasi (FAZA CRONICA)
Megacolon
SPOROZOARE
1. Toxoplasma gondii
Sporozoare tisulare
Toxoplasma gondii
Toxoplasma gondii
Stadiul de OOCHIST
Examen coproparazitologic fecale PISICA
P.C.
Sporochisti
Sporozoiti
uninucleati
Toxoplasma gondii
Stadiul de TACHIZOIT (trofozoitul de Toxoplasma)
TACHIZOITI uninucleati
Macrofag
tachys (gr)=rapid; zoon (gr)= animal
Frotiu
ascita
soarece
infectat
coloratie
Giemsa
Toxoplasma gondii
Stadiul de CHIST TISULAR cu bradizoiti
Celula
nervoasa
BRADIZOITI
bradys (gr)=lent; zoon (gr)= animal
Amprenta
tesut
cerebral
coloratie
Giemsa
Toxoplasma gondii
Neurotoxoplasmoza (la imunosupresati)
CT
Toxoplasma gondii
Corioretinita
(la imunosupresati si la
nou-nascuti cu
toxoplasmoza
congenitala)
Toxoplasma gondii
Hidrocefalie
2. Plasmodium vivax
Plasmodium ovale
Plasmodium malariae
Plasmodium falciparum
Sporozoare tisulare
Plasmodium sp.
Plasmodium sp.
Tantarul Anopheles sp.
Plasmodium sp.
OOCHISTI fixati pe fata externa a stomacului unui tantar infectat.
Plasmodium sp.
SPOROZOITI
Frotiu GLANDE SALIVARE TANTAR - coloratie GIEMSA
Plasmodium sp.
MEROZOITI INTRAHEPATOCITARI
Amprenta
tesut
hepatic
coloratie
Giemsa
Plasmodium sp.
Plasmodium vivax
Nucleu
inel
Citoplasma
putina,
periferica
Vacuola
centrala
INEL= TROFOZOIT TANAR
Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA
Plasmodium sp.
Plasmodium vivax
Citoplasma
bogata, cu
multe
prelungiri
Nucleu
amoeba
AMOEBA = TROFOZOIT MATUR
Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA
Plasmodium sp.
Plasmodium vivax
Nuclei
merozoiti
Granulatii
Schuffner
ROZETA = SCHIZONT MATUR
16-24 merozoiti
Corp muriform
Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA
Plasmodium sp.
Plasmodium vivax
GAMETOCIT MASCULIN
Nucleu
central
nedefinit
Granulatii
Schuffner
Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA
Plasmodium sp.
Plasmodium vivax
GAMETOCIT FEMININ
Nucleu gametocit -
periferic, triunghiular
Granulatii
Schuffner
Frotiu
SANGE
PERIFERIC
coloratie
GIEMSA
Plasmodium sp.
Plasmodium ovale
INEL= TROFOZOIT TANAR
Nucleu inel
Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA
Plasmodium sp.
Plasmodium ovale
Nucleu
amoeba
AMOEBA = TROFOZOIT MATUR
Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA
Plasmodium sp.
Plasmodium ovale
ROZETA = SCHIZONT MATUR
8-12 merozoiti
Nuclei
merozoiti
Frotiu
SANGE
PERIFERIC
coloratie
GIEMSA
Plasmodium sp.
Plasmodium ovale
Granulatii
Schuffner /
James
GAMETOCIT MASCULIN
Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA
Plasmodium sp.
Plasmodium ovale
Nucleu gametocit -
periferic, triunghiular
Granulatii
Schuffner /
James
GAMETOCIT FEMININ
Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA
Plasmodium sp.
Plasmodium malarie
INEL= TROFOZOIT TANAR
Nucleu
inel
Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA
Plasmodium sp.
Plasmodium malarie
Granulatii
Ziemann
Nucleu
amoeba
Amoeba in banda
AMOEBA = TROFOZOIT MATUR
Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA
Plasmodium sp.
Plasmodium malarie
Nuclei
granulocit
Nuclei
merozoiti
ROZETA = SCHIZONT MATUR
8-12 merozoiti
Rozeta in margareta
Granulatii
Ziemann
Examen
hematologic =
Picatura
groasa
SANGE
PERIFERIC
coloratie
GIEMSA
Plasmodium sp.
Plasmodium malarie
GAMETOCIT MASCULIN
Granulatii
Ziemann
Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA
Plasmodium sp.
Plasmodium malarie
GAMETOCIT FEMININ
Nucleu gametocit -
periferic, triunghiular
Granulatii
Ziemann
Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA
Plasmodium sp.
Plasmodium falciparum
INEL= TROFOZOIT TANAR
Inele uni si bi nucleate
Hematie
multiparazitata
cu inele
FAZA INCIPIENTA
Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA
Plasmodium sp.
Plasmodium falciparum
FAZA COMATOASA
AMOEBA = TROFOZOIT MATUR
Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA
Plasmodium sp.
Plasmodium falciparum
ROZETA = SCHIZONT MATUR
8-32 merozoiti
granulatii
Maurer
Merozoiti
uninucleati
FAZA COMATOASA
Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA
Plasmodium sp.
Plasmodium falciparum
GAMETOCIT MASCULIN in cosulet
granulatii
Maurer
Nucleu
gametocit
FAZA INCIPIENTA
Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA
Plasmodium sp.
Plasmodium falciparum
GAMETOCIT FEMININ in cosulet
FAZA INCIPIENTA
Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA
Plasmodium falciparum
Neuropaludism Malarie cerebrala
Cryptosporidium sp.
3. Cryptosporidium parvum
Cryptosporidium hominis
Sporozoare digestive
Cryptosporidium sp.
Stadiul de OOCHIST
Examen coproparazitologic = Frotiu fecale coloratie ZIEHL-NIELSEN modificata
Sporozoiti
uninucleati
(4)
4. Isospora belli
Sporozoar digestiv
Isospora belli
Isospora belli
Stadiul de OOCHIST
Frotiu fecale coloratie
ZIEHL-NIELSEN
modificata
Preparat
proaspat L+ l
din fecale cu SF
Qochist
matur
Qochist
imatur
Sporochist
Sporozoiti uninucleati
Examen coproparazitologic
5. Pneumocystis carinii
Pneumocystis jiroveci
Sporozoar - Transfug
Pneumocystis carinii Pneumocystis
jiroveci
Cale
aerogena
Pneumocystis carinii
TROFOZOITI (nuclei trofozoiti in nor cu puncte)
Frotiu LBA coloratie GIEMSA.
Pneumocystis carinii
CHISTI
cu (8)
corpusculii
colorati
TROFOZOITI
Frotiu LBA coloratie GIEMSA.
Pneumocystis carinii
Frotiu LBA coloratie Albastru de Toluidina.
CHISTI cu perete
celular colorat
Pneumocystis carinii
RX- plamani in geam mat