Medicina generala UMF Carol Davila

PROTOZOARE

Asistent universitar NEAGOE IONELA

AMIBE

AMIBE PATOGENE

Entamoeba hystolitica

Entamoeba hystolitica
Stadiul de CHIST

Examen coproparazitologic = Frotiu din fecale coloratie Tricrom

Entamoeba hystolitica
Trophe (gr)=
hranire; zoon
(gr)= animal

Examen
coproparazitologic =
Frotiu din fecale
muco-piosangvinolente
coloratie Giemsa

Stadiul de TROFOZOIT

Entamoeba hystolitica
Colonoscopie Abcese primare in buton de camasa

Entamoeba hystolitica
Abces primar in buton de camasa

Examen histopatologic = Sectiune tesut intestinal coloratie H&E

Entamoeba hystolitica
Abces secundar amoebian - hepatic

Entamoeba hystolitica
Abces secundar amoebian -perianal

AMIBE COMENSALE

Entamoeba coli
Stadiul de CHIST

Examen
coproparazitologic
= Preparat proaspat
L+l din fecale +
solutie Lugol

8 nuclei in
planuri
suprapuse

AMIBE LIBERE CONDITIONAT

PATOGENE
1.
2.

Naegleria fowlery
Acanthamoeba sp.

Naegleria fowlery

Naegleria fowlery
Stadiul de CHIST

Naegleria fowlery
Stadiul FLAGELAT

Flagel
(2)

Naegleria fowlery
Stadiul de TROFOZOIT

Recunoastere dupa nucleul cu aspect de tinta

Examen histopatologic =
Sectiune tesut cerebral
coloratie H&E

Acanthamoeba sp.

Acanthamoeba sp.
Stadiul de CHIST

Preparat
proaspat
L+l din
suspensie
tesut de
raclaj
corneean

Acanthamoeba sp.
Stadiul de TROFOZOIT

Nucleu

Preparat
proaspat
L+l din
LCR

Acantopodii

Acanthamoeba sp.
Keratita amoebiana

Acanthamoeba
sp.
Abcese subcutanate granulomatoase (la imunosupresati)

Acanthamoeba sp.
Encefalita granulomatoasa amoebiana cronica (la imunosupresati)

FLAGELATE

FLAGELATE CAVITARE
1.
2.

Giardia duodenalis
Trichomonas vaginalis

Giardia duodenalis

Giardia duodenalis
Stadiul de CHIST

Examen
coproparazitologic
= Preparat proaspat
L+l din fecale formate
+ SF

Giardia duodenalis
Stadiul de TROFOZOIT

Examen
coproparazitolo
gic =
Frotiu fecale
diareice
coloratie Giemsa

Giardia duodenalis
Stadiul de TROFOZOIT

Ventuza/Disc adeziv
Instalare MALABSORTII

Trichomonas vaginalis

Trichomonas vaginalis
Stadiul de TROFOZOIT

Frotiu secretie coloratie Giemsa

Trichomonas vaginalis
Coposcopie Vaginita + Leucoree FAZA INCIPIENTA

Trichomonas vaginalis
Colposcopie Strawberry cervix + coinfectie cu Candida

Trichomonas vaginalis

Testul
InPouch
TV pentru
detectia
directa si
cultivarea
trofozoitilor

Trichomonas vaginalis
Test rapid imunocromatografic detectie antigene

HEMOFLAGELATE
1.

Leishmania sp.

2.
3.

Trypanosoma brucei
Trypanosoma cruzii

Leishmania sp.

Leishmania sp.
(Sandfly)

Phlebotomus sp

Lutzomia sp.

Mastigot =
kinetida (gr) =
flagel+
corpuscul
bazal/kinetosom

Leishmania sp.
Stadiul PROMASTOGOT

Flagel liber

kinetoplast

Frotiu GLANDE
SALIVARE
MUSCULITA DE
NISIP - coloratie
GIEMSA

nucleu

Leishmania sp.
Stadiul AMASTOGOT

Amastigot uninucleat

kinetoplast
Frotiu aspirat
medular
coloratie
Giemsa

Macrofag

Leishmania sp.
Buton de Orient
- Forma uscata - L. tropica

Leishmania sp.
Buton Orient - Forma umeda L. major

Leishmania sp.
Forma ESPUNDIA L. brasiliensis

Leishmania sp.
Ulcerul chiclero- L. mexicana

Leishmania sp.

Boala Kala-azar, febra Dum-Dum, boala neagra FAZA CRONICA

L. donovani
L. Infantum
L. chagasi

Leishmanioza cutanata post kala-azar FAZA CRONICA

Leishmania sp.
Leismania donovani

Trypanosoma brucei

Trypanosoma brucei
Glossina sp. ( musca tze-tze )

Trypanosoma brucei
Stadiul EPIMASTOGOT

Membrana ondulanta
scurta
Frotiu din
cultura
dezvoltata
pe mediul
NNN coloratie
GIEMSA

Flagel
liber
anterior

Nucleu

Trypanosoma brucei
Stadiul TRIPOMASTIGOT

Membrana
ondulanta lunga

Corpuscul bazal /Kinetosom

Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA

Kinetoplast

Nucleu

Flagel liber
anterior

Trypanosoma brucei gambiense

Sleeping sickness (Boala somnului) FAZA INCIPIENTA

Semnul Winterbottom

Trypanosoma brucei
Sleeping sickness (Boala somnului) FAZA CRONICA

Meningoencefalita cronica

Trypanosoma cruzii

Trypanosoma cruzii

Triatoma sp. (Kissing bug)

Reduviid bug

Trypanosoma cruzii
Stadiul TRIPOMASTIGOT

Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA

Trypanosoma cruzii
Stadiul AMASTOGOT

Amastogoti
uninucleati

Celula
nervoasa

Aprenta tesut cardiac

coloratie Giemsa

Trypanosoma cruzii
Boala Chagasi (FAZA INCIPIENTA)

Semnul Romana

Trypanosoma cruzii
Boala Chagasi (FAZA CRONICA)

Cardiomiopatie cu
megalie cardiaca

Anevrism
cardiac

Trypanosoma cruzii
Boala Chagasi (FAZA CRONICA)

Megacolon

SPOROZOARE

Sporozoare tisulare
1. Toxoplasma gondii

Toxoplasma gondii

Toxoplasma gondii
Stadiul de OOCHIST
P.C.
Sporozoiti
uninucleati

Sporochisti

Examen coproparazitologic fecale PISICA

Toxoplasma gondii
Stadiul de TACHIZOIT (trofozoitul de Toxoplasma)

Frotiu
ascita
soarece
infectat
coloratie
Giemsa

TACHIZOITI uninucleati

Macrofag
tachys (gr)=rapid; zoon (gr)= animal

Toxoplasma gondii
Stadiul de CHIST TISULAR cu bradizoiti
Celula
nervoasa

BRADIZOITI

bradys (gr)=lent; zoon (gr)= animal

Amprenta
tesut
cerebral
coloratie
Giemsa

Toxoplasma
gondii
Neurotoxoplasmoza (la imunosupresati)

CT

Toxoplasma gondii
Corioretinita
(la imunosupresati si la
nou-nascuti cu
toxoplasmoza
congenitala)

Toxoplasma gondii
Hidrocefalie

Sporozoare tisulare
2. Plasmodium vivax
Plasmodium ovale
Plasmodium malariae
Plasmodium falciparum

Plasmodium sp.

Plasmodium sp.
Tantarul Anopheles sp.

Plasmodium sp.
OOCHISTI fixati pe fata externa a stomacului unui tantar infectat.

Plasmodium sp.
SPOROZOITI

Frotiu GLANDE SALIVARE TANTAR - coloratie GIEMSA

Plasmodium sp.
Amprenta
tesut
hepatic
coloratie
Giemsa

MEROZOITI INTRAHEPATOCITARI

Plasmodium sp.
Plasmodium vivax

Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA

INEL= TROFOZOIT TANAR

Citoplasma
putina,
periferica

Nucleu
inel

Vacuola
centrala

Plasmodium sp.
Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA

Plasmodium vivax
AMOEBA = TROFOZOIT MATUR

Citoplasma
bogata, cu
multe
prelungiri

Nucleu
amoeba

Plasmodium sp.
Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA

Plasmodium vivax
ROZETA = SCHIZONT MATUR
16-24 merozoiti
Granulatii
Schuffner

Nuclei
merozoiti

Corp muriform

Plasmodium sp.
Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA

Plasmodium vivax
GAMETOCIT MASCULIN

Granulatii
Schuffner

Nucleu
central
nedefinit

Plasmodium sp.
Plasmodium vivax
GAMETOCIT FEMININ
Granulatii
Schuffner
Frotiu
SANGE
PERIFERIC
coloratie
GIEMSA
Nucleu gametocit periferic, triunghiular

Plasmodium sp.
Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA

Plasmodium ovale
INEL= TROFOZOIT TANAR

Nucleu inel

Plasmodium sp.
Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA

Plasmodium ovale
AMOEBA = TROFOZOIT MATUR

Nucleu
amoeba

Plasmodium
sp.
Plasmodium ovale
ROZETA = SCHIZONT MATUR
8-12 merozoiti

Frotiu
SANGE
PERIFERIC
coloratie
GIEMSA
Nuclei
merozoiti

Plasmodium sp.
Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA

Plasmodium ovale

GAMETOCIT MASCULIN

Granulatii
Schuffner /
James

Plasmodium sp.
Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA

Plasmodium ovale
GAMETOCIT FEMININ
Granulatii
Schuffner /
James

Nucleu gametocit periferic, triunghiular

Plasmodium sp.
Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA

Plasmodium malarie
INEL= TROFOZOIT TANAR

Nucleu
inel

Plasmodium sp.
Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA

Plasmodium malarie
AMOEBA = TROFOZOIT MATUR

Amoeba in banda
Granulatii
Ziemann

Nucleu
amoeba

Plasmodium sp.
Plasmodium malarie
ROZETA = SCHIZONT MATUR
8-12 merozoiti
Rozeta in margareta
Examen
hematologic =
Picatura
groasa
SANGE
PERIFERIC
coloratie
GIEMSA

Nuclei
merozoiti

Granulatii
Ziemann
Nuclei
granulocit

Plasmodium sp.
Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA

Plasmodium malarie
GAMETOCIT MASCULIN

Granulatii
Ziemann

Plasmodium sp.
Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA

Plasmodium malarie
GAMETOCIT FEMININ

Granulatii
Ziemann

Nucleu gametocit periferic, triunghiular

Plasmodium sp.
Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA

Plasmodium falciparum
INEL= TROFOZOIT TANAR

Inele uni si bi nucleate

Hematie
multiparazitata
cu inele

FAZA INCIPIENTA

Plasmodium sp.
Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA

Plasmodium falciparum
AMOEBA = TROFOZOIT MATUR

FAZA COMATOASA

Plasmodium sp.
Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA

Plasmodium falciparum
ROZETA = SCHIZONT MATUR
8-32 merozoiti

Merozoiti
uninucleati

granulatii
Maurer
FAZA COMATOASA

Plasmodium sp.
Plasmodium falciparum
Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA

GAMETOCIT MASCULIN in cosulet

Nucleu
gametocit

FAZA INCIPIENTA

granulatii
Maurer

Plasmodium
sp.
Plasmodium falciparum
Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA

GAMETOCIT FEMININ in cosulet

FAZA INCIPIENTA

Plasmodium
falciparum
Neuropaludism Malarie cerebrala

Cryptosporidium sp.

Sporozoare digestive
3. Cryptosporidium parvum
Cryptosporidium hominis

Cryptosporidium sp.
Stadiul de OOCHIST

Sporozoiti
uninucleati
(4)

Examen coproparazitologic = Frotiu fecale coloratie ZIEHL-NIELSEN modificata

Sporozoar digestiv
4. Isospora belli

Isospora belli

Isospora belli
Stadiul de OOCHIST
Examen coproparazitologic
Qochist
matur

Qochist
imatur
Sporozoiti uninucleati

Sporochist

Preparat
proaspat L+ l
din fecale cu SF

Frotiu fecale coloratie

ZIEHL-NIELSEN
modificata

Sporozoar - Transfug
5. Pneumocystis carinii
Pneumocystis jiroveci

Pneumocystis carinii Pneumocystis

jiroveci
Cale
aerogena

Pneumocystis carinii

TROFOZOITI (nuclei trofozoiti in nor cu puncte)

Frotiu LBA coloratie GIEMSA.

Pneumocystis carinii

TROFOZOITI
CHISTI
cu (8)
corpusculii
colorati

Frotiu LBA coloratie GIEMSA.

Pneumocystis carinii
CHISTI cu perete
celular colorat

Frotiu LBA coloratie Albastru de Toluidina.

Pneumocystis carinii

RX- plamani in geam mat