TESTUL

COAGULAZEI
PREZENTARE REALIZATA DE GAIANU IOANA-ELIZA, GURPA 45, SERIA 4

Coagulaza este o protein-enzima produsa

de anumite microorganisme.
Ea da posibilitea microorganismului sa
converteasca fibrinogenul solubil la
fibrina insolubila.
In laborator, e folosita pentru a diferentia
intre tipurile de stafilococi-coagulazopozitivi ori negativi.

Coagulaza

poate fi de 2 feluri
1.legata de stafilococ
2.eliberata in mediu

 Coagulaza

reactioneaza cu
protrombina din sange, complexul
format numindu-se stafilotrombina,
declansand astfel conversia
fibrinogenului in fibrina insolubila.

stafilotromabina

In addition, most strains express

a fibrin/fibrinogen binding protein
(clumping factor-factor de
atasare) which promotes
attachment to blood clots and
traumatized tissue.

Coagulase is tightly bound to the surface

of the bacteriumS. aureusand can coat
its surface with fibrin upon contact with
blood. The fibrin clot may protect the
bacterium from phagocytosis and isolate
it from other defenses of the host. It has
been proposed that fibrin-coated
staphylococci resist phagocytosis,
making the bacteria more virulent.

Cum deosebim coagulaza legata

de cea libera?
Pe

cea legata o testam pe lame

Desigur vom avea intotdeauna probe de control pentru a exclude preveni

autoaglutinarea plasmei(control negativ)

Si putem avea si o proba cu Stafilococ coagulazo pozitiv ca martor.(control pozitiv)

Cea

libera va fi testata in tuburi.